Ptychodera Cisreg Development: pairwise species comparisons (BLAST reciprocal hits and Orthofinder orthogroups)
Alberto Perez-Posada
4/22/2023
About
In this markdown, we will do a comparison of the transcriptomes during development of Ptychodera, Amphixous, and Purple Sea Urchin.
For this, we will use a solution of custom R functions and wrappers that we have named “comparABle” (kudos to @agilgal for the name!).
Load libraries
library(tidyverse)
library(stringr)
library(BiocGenerics)
library(EDASeq)
library(DESeq2)
library(tximport)
library(limma)
library(ComplexHeatmap)
library(circlize)
library(RColorBrewer)
require(data.table)
require(stringr)
require(tidytree)
require(ggtree)
require(rvcheck)
require(treeio)
require(dendextend)
require(phangorn)
require(phytools)
require(ComplexHeatmap)
require(foreach)
require(doParallel)
require(colorspace)
library(topGO)Load functions
source("code/r_code/r_functions/sourcefolder.R")
sourceFolder(
"code/r_code/r_functions",
recursive = TRUE
)
sourceFolder(
"code/r_code/r_general",
recursive = TRUE
)Load Data
We will first load the data of our three species:
# Ptychodera analyses
load("outputs/rda/deseq2.rda")
# Amphioxus reanalysis
load("outputs/rda/blan_reanalysis.rda")
# Purple Sea Urchin reanalysis
load("outputs/rda/spur_reanalysis.rda")We will follow by loading all the gene family data that we need. This is the gene/gfam lookup table (extracted from the OrthoXML output file from OMA) and the respective table to transform the gene indexes into the actual gene id of every species.
gene_gfam <-
read.delim2(
file = "outputs/comparative/20240404_orthofinder/Orthogroups_melt.tsv",
header = TRUE
)This gene_gfam file will be used later on as proxy to compare developmental stages.
head(gene_gfam)## id gfam
## 1 AENJA_00110 OG0000000
## 2 AENJA_00111 OG0000000
## 3 AENJA_01531 OG0000000
## 4 AENJA_01537 OG0000000
## 5 AENJA_01755 OG0000000
## 6 AENJA_05264 OG0000000OF_pfla_blan <- read.delim2("outputs/comparative/20240404_orthofinder/one_to_one_orthologues/1to1_pfla_blan.tsv", header = TRUE)
OF_pfla_spur <- read.delim2("outputs/comparative/20240404_orthofinder/one_to_one_orthologues/1to1_pfla_spur.tsv", header = TRUE)load("outputs/rda/geneage.rda")For more functional annotation, we will use the COG functional categories and the GO terms of Ptychodera.
pfla_cogs <- read.table(
"outputs/functional_annotation/COGs/pfla_cogs.tsv",
col.names = c("id","cog")
)
# GO terms
pfla_id2go <-
readMappings(
"outputs/functional_annotation/go_blast2go/GO_annotation.txt"
)Ptychodera and Amphioxus
We will first compare the transcriptomes and stage-specific clusters of Ptychodera and Amphioxus. For this, we will define a number of objects that will enter into our custom wrapper function comparABle. The most important of them being:
- Objects a and b are the expression profile datasets (gene x condition/stage/etc) of species A and B.
- Object o is a 1-to-1 orthologue association file between genes of species A and B (e.g. BLAST reciprocal best hits, OMA 1:1 orthologues, …)
- Object f is a “gene family”/orthogroup file associating genes from species A and B to “gene families”
- Objects ma, mb are a gene classification system. For each species a and b, a file associating genes to gene moules (e.g. k-means clusters, hierarchical clusters, WGCNA modules, etc.)
- Object g is a gene age file for the gene families, and the genes, of each species.
In addition, we will also pass it a list of gene ontologies, a COG functional category association file, and a list of gene ages that are shared between species A and B.
# Expression data
a = pfla_rna_counts
b = blan_counts
# Samples for rowmeans_by repl
a_samples = levels(condition_x)
b_samples = unique(sub("_.$", "", colnames(b)))
# Family/orthology data
o = unique(OF_pfla_blan)
f = gene_gfam[grep("TCONS|BRALA",gene_gfam$id),]
f$id <- gsub("\\.p[0-9]+","",f$id)
# Module/Cluster information
ma = data.frame(
id = rownames(pfla_rna_dev),
module = pfla_rna_dev$cID
)
mb = blan_cl
colnames(mb) <- c("id","module")
# Gene Age
ga = pfla_age[,c(1,3)]
colnames(ga) = c("id","age")
# COG
cog_a <- pfla_cogs
# GOs
a_universe = rownames(vsd_allgen)
a_id2go = pfla_id2go
# Common Evo Nodes
common_evo_nodes = unique(ga$age)[!(unique(ga$age) %in% c("6_Ambul","7_Hemich","8_Pfla"))]Below we will run the comparABle wrapper. Briefly, what this wrapper does is:
- Normalise the expression datasets
- Associate the datasets using the association file of 1-to-1 orthologues
- Perform a principal component analysis with the merge of the two datasets
- Perform pairwise correlations and distance metrics between conditions (in our case, developmental stages) of datasets to see their relationships of similarity. By default: Pearson Correlation, Spearman Correlation, and Jensen-Shannon Divergence.
- Identify the genes showcasing highes values of correlations across a number of pre-determined conditions (in our case, stages), and perform Gene Age and Gene Ontology enrichment analysis
- Perform a co-occurrence analysis in the merge of the two datasets (pairwise correlations, hierarchical clustering, and bootstrapping with downsampling)
- Perform Orthology Overlap analysis (hypergeometric and binomial) in pairwise correlations between gene sets (==stage-specific clusters) of each species.
- Identify the genes of the gene families that appear significantly enriched across pairs of gene sets, and perform Gene Age, Gene Ontology, and Functional Category enrichment analyses.
This can take some time.
PFLA_BLAN_COMPARISON_OF <- comparABle(
a_name = "P.flava",
b_name = "B.lanceolatum",
a = a,
b = b,
o = o,
f = f,
ma = ma,
mb = mb,
ga = ga,
gb = gb,
cog_a = cog_a,
cog_b = cog_b,
a_samples = a_samples,
b_samples = b_samples,
highlyvariable = FALSE,
cooc_p = 0.05,
cooc_h = c(0.70,0.95),
cooc_cor_method = "pearson",
a_universe = a_universe,
a_id2go = a_id2go,
common_evo_nodes = common_evo_nodes,
sep = ",\ "
)## [1] "Tidy up data"## Loading required package: preprocessCore## [1] "Merge data"
## [1] "Correlations"
## [1] "PCA"
## [1] "Co-Occurrence"
## [1] "Common genes in Correlations"## Loading required package: ggpointdensity## The top five similar pair of stages are: 19.2192 19.45585 19.6026 19.67682 19.74663
## [1] "Subsetting count matrices"
## [1] "Model fitting and top genes"## Loading required package: edgeR## [1] "Common genes in Correlations (GO)"
## [1] "Starting analysis 1 of 5"##
## Building most specific GOs .....## ( 15540 GO terms found. )##
## Build GO DAG topology ..........## ( 15584 GO terms and 36018 relations. )##
## Annotating nodes ...............## ( 15464 genes annotated to the GO terms. )##
## -- Classic Algorithm --
##
## the algorithm is scoring 3284 nontrivial nodes
## parameters:
## test statistic: fisher## [1] "Starting analysis 2 of 5"##
## Building most specific GOs .....## ( 15540 GO terms found. )##
## Build GO DAG topology ..........## ( 15584 GO terms and 36018 relations. )##
## Annotating nodes ...............## ( 15464 genes annotated to the GO terms. )##
## -- Classic Algorithm --
##
## the algorithm is scoring 3072 nontrivial nodes
## parameters:
## test statistic: fisher## [1] "Starting analysis 3 of 5"##
## Building most specific GOs .....## ( 15540 GO terms found. )##
## Build GO DAG topology ..........## ( 15584 GO terms and 36018 relations. )##
## Annotating nodes ...............## ( 15464 genes annotated to the GO terms. )##
## -- Classic Algorithm --
##
## the algorithm is scoring 3007 nontrivial nodes
## parameters:
## test statistic: fisher## [1] "Starting analysis 4 of 5"##
## Building most specific GOs .....## ( 15540 GO terms found. )##
## Build GO DAG topology ..........## ( 15584 GO terms and 36018 relations. )##
## Annotating nodes ...............## ( 15464 genes annotated to the GO terms. )##
## -- Classic Algorithm --
##
## the algorithm is scoring 3270 nontrivial nodes
## parameters:
## test statistic: fisher## [1] "Starting analysis 5 of 5"##
## Building most specific GOs .....## ( 15540 GO terms found. )##
## Build GO DAG topology ..........## ( 15584 GO terms and 36018 relations. )##
## Annotating nodes ...............## ( 15464 genes annotated to the GO terms. )##
## -- Classic Algorithm --
##
## the algorithm is scoring 2826 nontrivial nodes
## parameters:
## test statistic: fisher## [1] "Common genes in Correlations (age)"
## [1] "Pairwise Orthology Overlap Strategy across modules -- hypergeometric and binonmial tests"
## [1] "Getting info on genes from shared families across modules"
## [1] "Starting analysis 1 of 20"##
## Building most specific GOs .....## ( 15540 GO terms found. )##
## Build GO DAG topology ..........## ( 15584 GO terms and 36018 relations. )##
## Annotating nodes ...............## ( 15464 genes annotated to the GO terms. )##
## -- Classic Algorithm --
##
## the algorithm is scoring 4148 nontrivial nodes
## parameters:
## test statistic: fisher## [1] "Starting analysis 2 of 20"##
## Building most specific GOs .....## ( 15540 GO terms found. )##
## Build GO DAG topology ..........## ( 15584 GO terms and 36018 relations. )##
## Annotating nodes ...............## ( 15464 genes annotated to the GO terms. )##
## -- Classic Algorithm --
##
## the algorithm is scoring 4441 nontrivial nodes
## parameters:
## test statistic: fisher## [1] "Starting analysis 3 of 20"##
## Building most specific GOs .....## ( 15540 GO terms found. )##
## Build GO DAG topology ..........## ( 15584 GO terms and 36018 relations. )##
## Annotating nodes ...............## ( 15464 genes annotated to the GO terms. )##
## -- Classic Algorithm --
##
## the algorithm is scoring 3954 nontrivial nodes
## parameters:
## test statistic: fisher## [1] "Starting analysis 4 of 20"##
## Building most specific GOs .....## ( 15540 GO terms found. )##
## Build GO DAG topology ..........## ( 15584 GO terms and 36018 relations. )##
## Annotating nodes ...............## ( 15464 genes annotated to the GO terms. )##
## -- Classic Algorithm --
##
## the algorithm is scoring 3699 nontrivial nodes
## parameters:
## test statistic: fisher## [1] "Starting analysis 5 of 20"##
## Building most specific GOs .....## ( 15540 GO terms found. )##
## Build GO DAG topology ..........## ( 15584 GO terms and 36018 relations. )##
## Annotating nodes ...............## ( 15464 genes annotated to the GO terms. )##
## -- Classic Algorithm --
##
## the algorithm is scoring 3379 nontrivial nodes
## parameters:
## test statistic: fisher## [1] "Starting analysis 6 of 20"##
## Building most specific GOs .....## ( 15540 GO terms found. )##
## Build GO DAG topology ..........## ( 15584 GO terms and 36018 relations. )##
## Annotating nodes ...............## ( 15464 genes annotated to the GO terms. )##
## -- Classic Algorithm --
##
## the algorithm is scoring 2909 nontrivial nodes
## parameters:
## test statistic: fisher## [1] "Starting analysis 7 of 20"##
## Building most specific GOs .....## ( 15540 GO terms found. )##
## Build GO DAG topology ..........## ( 15584 GO terms and 36018 relations. )##
## Annotating nodes ...............## ( 15464 genes annotated to the GO terms. )##
## -- Classic Algorithm --
##
## the algorithm is scoring 2661 nontrivial nodes
## parameters:
## test statistic: fisher## [1] "Starting analysis 8 of 20"##
## Building most specific GOs .....## ( 15540 GO terms found. )##
## Build GO DAG topology ..........## ( 15584 GO terms and 36018 relations. )##
## Annotating nodes ...............## ( 15464 genes annotated to the GO terms. )##
## -- Classic Algorithm --
##
## the algorithm is scoring 2186 nontrivial nodes
## parameters:
## test statistic: fisher## [1] "Starting analysis 9 of 20"##
## Building most specific GOs .....## ( 15540 GO terms found. )##
## Build GO DAG topology ..........## ( 15584 GO terms and 36018 relations. )##
## Annotating nodes ...............## ( 15464 genes annotated to the GO terms. )##
## -- Classic Algorithm --
##
## the algorithm is scoring 1816 nontrivial nodes
## parameters:
## test statistic: fisher## [1] "Starting analysis 10 of 20"##
## Building most specific GOs .....## ( 15540 GO terms found. )##
## Build GO DAG topology ..........## ( 15584 GO terms and 36018 relations. )##
## Annotating nodes ...............## ( 15464 genes annotated to the GO terms. )##
## -- Classic Algorithm --
##
## the algorithm is scoring 2892 nontrivial nodes
## parameters:
## test statistic: fisher## [1] "Starting analysis 11 of 20"##
## Building most specific GOs .....## ( 15540 GO terms found. )##
## Build GO DAG topology ..........## ( 15584 GO terms and 36018 relations. )##
## Annotating nodes ...............## ( 15464 genes annotated to the GO terms. )##
## -- Classic Algorithm --
##
## the algorithm is scoring 3002 nontrivial nodes
## parameters:
## test statistic: fisher## [1] "Starting analysis 12 of 20"##
## Building most specific GOs .....## ( 15540 GO terms found. )##
## Build GO DAG topology ..........## ( 15584 GO terms and 36018 relations. )##
## Annotating nodes ...............## ( 15464 genes annotated to the GO terms. )##
## -- Classic Algorithm --
##
## the algorithm is scoring 2482 nontrivial nodes
## parameters:
## test statistic: fisher## [1] "Starting analysis 13 of 20"##
## Building most specific GOs .....## ( 15540 GO terms found. )##
## Build GO DAG topology ..........## ( 15584 GO terms and 36018 relations. )##
## Annotating nodes ...............## ( 15464 genes annotated to the GO terms. )##
## -- Classic Algorithm --
##
## the algorithm is scoring 3610 nontrivial nodes
## parameters:
## test statistic: fisher## [1] "Starting analysis 14 of 20"##
## Building most specific GOs .....## ( 15540 GO terms found. )##
## Build GO DAG topology ..........## ( 15584 GO terms and 36018 relations. )##
## Annotating nodes ...............## ( 15464 genes annotated to the GO terms. )##
## -- Classic Algorithm --
##
## the algorithm is scoring 2647 nontrivial nodes
## parameters:
## test statistic: fisher## [1] "Starting analysis 15 of 20"##
## Building most specific GOs .....## ( 15540 GO terms found. )##
## Build GO DAG topology ..........## ( 15584 GO terms and 36018 relations. )##
## Annotating nodes ...............## ( 15464 genes annotated to the GO terms. )##
## -- Classic Algorithm --
##
## the algorithm is scoring 1409 nontrivial nodes
## parameters:
## test statistic: fisher## [1] "Starting analysis 16 of 20"##
## Building most specific GOs .....## ( 15540 GO terms found. )##
## Build GO DAG topology ..........## ( 15584 GO terms and 36018 relations. )##
## Annotating nodes ...............## ( 15464 genes annotated to the GO terms. )##
## -- Classic Algorithm --
##
## the algorithm is scoring 3158 nontrivial nodes
## parameters:
## test statistic: fisher## [1] "Starting analysis 17 of 20"##
## Building most specific GOs .....## ( 15540 GO terms found. )##
## Build GO DAG topology ..........## ( 15584 GO terms and 36018 relations. )##
## Annotating nodes ...............## ( 15464 genes annotated to the GO terms. )##
## -- Classic Algorithm --
##
## the algorithm is scoring 3079 nontrivial nodes
## parameters:
## test statistic: fisher## [1] "Starting analysis 18 of 20"##
## Building most specific GOs .....## ( 15540 GO terms found. )##
## Build GO DAG topology ..........## ( 15584 GO terms and 36018 relations. )##
## Annotating nodes ...............## ( 15464 genes annotated to the GO terms. )##
## -- Classic Algorithm --
##
## the algorithm is scoring 1957 nontrivial nodes
## parameters:
## test statistic: fisher## [1] "Starting analysis 19 of 20"##
## Building most specific GOs .....## ( 15540 GO terms found. )##
## Build GO DAG topology ..........## ( 15584 GO terms and 36018 relations. )##
## Annotating nodes ...............## ( 15464 genes annotated to the GO terms. )##
## -- Classic Algorithm --
##
## the algorithm is scoring 2010 nontrivial nodes
## parameters:
## test statistic: fisher## [1] "Starting analysis 20 of 20"##
## Building most specific GOs .....## ( 15540 GO terms found. )##
## Build GO DAG topology ..........## ( 15584 GO terms and 36018 relations. )##
## Annotating nodes ...............## ( 15464 genes annotated to the GO terms. )##
## -- Classic Algorithm --
##
## the algorithm is scoring 3061 nontrivial nodes
## parameters:
## test statistic: fisher## [1] "Plots"
## [1] "Generating results"
After running this we can start exploring the comparisons. First we will tidy up the pairwise correlation data and add names to the rows and columns of the jensen-shannon distance matrix.
Here are the heatmaps of pairwise correlations between Ptychodera and Amphioxus.
plot_cors(PFLA_BLAN_COMPARISON_OF$pairwise_correlations)
A quick sanity check that this is consistently observed regardles of genes by doing some bootstraping and checkin the mean observed JSD:
js_mean <- jsd_with_subsampling(
a_o = PFLA_BLAN_COMPARISON_OF$merged_data$a_o ,
b_o = PFLA_BLAN_COMPARISON_OF$merged_data$b_o,
n = 1000, p = 0.25
)
#relativise to min and max values
js_mean_rel <- relativise(js_mean$mean)
h3_avg_rel <- Heatmap(js_mean_rel,cluster_rows = F, cluster_columns = F, show_row_names = TRUE, name = "JSD", col = brewer.pal(10,"RdBu"))
draw(h3_avg_rel)
Here is the average expression profile of genes more expressed in the most similar stages between Ptychodera an Amphioxus, including GO terms and age enrichment:
# Common genes, highly correlated
p1 <- plot_hicor_genes(
scatter = PFLA_BLAN_COMPARISON_OF$high_corr_genes$pairwise_data$hicor_topgenes[[1]]$plot_topgenes,
GO_table = PFLA_BLAN_COMPARISON_OF$high_corr_genes$GOs$GOtable[[1]],
age_table = PFLA_BLAN_COMPARISON_OF$high_corr_genes$age[[1]]$AgeperModule,
age_enrichemnt_hm = PFLA_BLAN_COMPARISON_OF$high_corr_genes$age[[1]]$heatmap
)
p2 <- plot_hicor_genes(
scatter = PFLA_BLAN_COMPARISON_OF$high_corr_genes$pairwise_data$hicor_topgenes[[2]]$plot_topgenes,
GO_table = PFLA_BLAN_COMPARISON_OF$high_corr_genes$GOs$GOtable[[2]],
age_table = PFLA_BLAN_COMPARISON_OF$high_corr_genes$age[[2]]$AgeperModule,
age_enrichemnt_hm = PFLA_BLAN_COMPARISON_OF$high_corr_genes$age[[2]]$heatmap
)
p3 <- plot_hicor_genes(
scatter = PFLA_BLAN_COMPARISON_OF$high_corr_genes$pairwise_data$hicor_topgenes[[3]]$plot_topgenes,
GO_table = PFLA_BLAN_COMPARISON_OF$high_corr_genes$GOs$GOtable[[3]],
age_table = PFLA_BLAN_COMPARISON_OF$high_corr_genes$age[[3]]$AgeperModule,
age_enrichemnt_hm = PFLA_BLAN_COMPARISON_OF$high_corr_genes$age[[3]]$heatmap
)
plot_grid(p1,p2,p3,ncol = 1)
Here is the co-occurrence analysis showcasing the Amphioxus stages most similar to Ptychodera gastrulation are the stages corresponding to neurulation.
Heatmap(
name = "co-occurrence",
PFLA_BLAN_COMPARISON_OF$coocurrence_analysis$cooccurrence[1:16,17:28],
cluster_rows = F,
cluster_columns = F,
col = sequential_hcl(10,"YlOrRd", rev = TRUE)
)
Here the orthology overlap strategy showcasing similarities at the gene family usage between hemichordate larval stages and amphioxus post-gastrulation development
pf_avg_hm+
PFLA_BLAN_COMPARISON_OF$plots$orthology_overlap_binomial_hm+
PFLA_BLAN_COMPARISON_OF$plots$orthology_overlap_hypgeom_hm
Here is a quick overview of the stats of the hypergeometric and binomial tests for pairwise comparisons:
summary(PFLA_BLAN_COMPARISON_OF$orthology_overlap_modules$pairwise_module_comparison$stats)## module_a module_b success_in_samples sample_size
## Length:550 Length:550 Length:550 Length:550
## Class :character Class :character Class :character Class :character
## Mode :character Mode :character Mode :character Mode :character
##
##
##
## success_in_pop gene_POP hypgeom_pval hypgeom_log
## Length:550 Length:550 Min. :0.0000000 Min. : 0.000
## Class :character Class :character 1st Qu.:0.0000000 1st Qu.: 1.621
## Mode :character Mode :character Median :0.0007268 Median : 7.228
## Mean :0.1647946 Mean : 12.234
## 3rd Qu.:0.1976050 3rd Qu.: 17.128
## Max. :1.0000000 Max. :226.377
## binom_pval gfams_common gfams_excl_a gfams_excl_b
## Min. :0.0000000 Length:550 Length:550 Length:550
## 1st Qu.:0.0000001 Class :character Class :character Class :character
## Median :0.0007450 Mode :character Mode :character Mode :character
## Mean :0.1413739
## 3rd Qu.:0.1368170
## Max. :1.0000000These is a quick overview at the number of genes in enriched gene families between pairs of comparisons:
# Group by module and summarize
commongenes <- PFLA_BLAN_COMPARISON_OF$orthology_overlap_modules$
genes_in_common_fams$commonfams$table_a_common %>%
group_by(module) %>%
summarize(
numgenes = n(),
genes = paste(ifelse(n() > 3, paste0(paste(head(id, 3), collapse = ",")," ..."), paste(id, collapse = ", ")), collapse = ", ")
)
# Rename columns
colnames(commongenes) <- c("module", "numgenes", "genes")
head(commongenes)## # A tibble: 6 × 3
## module numgenes genes
## <chr> <int> <chr>
## 1 02__3 162 TCONS_00031538,TCONS_00032505,TCONS_00022997 ...
## 2 02__6 162 TCONS_00023197,TCONS_00023330,TCONS_00024839 ...
## 3 03__8 124 TCONS_00024709,TCONS_00024711,TCONS_00023765 ...
## 4 06__6 284 TCONS_00038700,TCONS_00038727,TCONS_00041502 ...
## 5 14__18 211 TCONS_00039151,TCONS_00039158,TCONS_00038525 ...
## 6 15__21 238 TCONS_00032648,TCONS_00038402,TCONS_00038444 ...And here the combination of gene age and functional categories enrichment:
PFLA_BLAN_COMPARISON_OF$orthology_overlap_modules$
genes_in_common_fams$commonfams$
age_a_common$heatmap +
PFLA_BLAN_COMPARISON_OF$orthology_overlap_modules$
genes_in_common_fams$commonfams$
cog_a_comon$heatmap
And here the gene ontology enrichment of enriched gene families between pairs of modules.
# Add a new column with the name of the original data frame
keygenes_commonfams_GO <-
bind_rows(
PFLA_BLAN_COMPARISON_OF$orthology_overlap_modules$
genes_in_common_fams$commonfams$go_a_common$GOtable,
.id = "pair_gfams"
)
# Rename the new column
colnames(keygenes_commonfams_GO)[1] <- "pair_gfams"
keygenes_commonfams_GO$logp <-
-log10(as.numeric(keygenes_commonfams_GO$classicFisher))
keygenes_commonfams_GO$logp[is.na(keygenes_commonfams_GO$logp)] <-
max(keygenes_commonfams_GO$logp, na.rm = T)
keygenes_commonfams_GO$obsexp <-
as.numeric(keygenes_commonfams_GO$Significant/keygenes_commonfams_GO$Expected)
keygenes_commonfams_GO <-
keygenes_commonfams_GO[
order(
keygenes_commonfams_GO$pair_gfams,
keygenes_commonfams_GO$Term
),
]
keygenes_commonfams_GO$Term <-
factor(
keygenes_commonfams_GO$Term,
levels = unique(keygenes_commonfams_GO$Term)
)The GO plot here:
gg <- keygenes_commonfams_GO %>%
ggplot(
aes(x=pair_gfams, y = Term, size = Significant, color = logp)
) + geom_point() +
theme_bw() +
theme(
axis.text.x = element_text(angle = 45, hjust = 1, size = 8 )
)+
theme(
axis.text.y.left = element_text(hjust = 1, size = 8 ),
legend.title=element_text(size=8 ),
legend.text=element_text(size=8 )
)+
scale_size_continuous(
name = "Genes affected",
range = c(2,6)
) + scale_color_viridis("-log10(P-value)") +
xlab("")+ylab("")
#ggPtychodera and Purple Sea Urchin
We will proceed the same as before using sea urchin in this case. Again, we define all the data necessary for the comparABle wrapper:
# Expression data
a = pfla_rna_counts
b = spur_vsd
# Samples for rowmeans_by repl
a_samples = levels(condition_x)
b_samples = unique(sub("_.$", "", colnames(b)))
# Family/orthology data
o = unique(OF_pfla_spur)
f = gene_gfam[grep("TCONS|STRPU",gene_gfam$id),]
f$id <- gsub("\\.p[0-9]+","",f$id)
# Module/Cluster information
ma = data.frame(
id = rownames(pfla_rna_dev),
module = pfla_rna_dev$cID
)
mb = spur_cl
colnames(mb) <- c("id","module")
# Gene Age
ga = pfla_age[,c(1,3)]
colnames(ga) = c("id","age")
# COG
cog_a <- pfla_cogs
# GOs
a_universe = rownames(vsd_allgen)
a_id2go = pfla_id2go
# Common Evo Nodes
common_evo_nodes = unique(ga$age)[!(unique(ga$age) %in% c("7_Hemich","8_Pfla"))]And we run comparABle:
PFLA_SPUR_COMPARISON_OF <- comparABle(
a_name = "P.flava",
b_name = "S.purpuratus",
a = a,
b = b,
o = o,
f = f,
ma = ma,
mb = mb,
ga = ga,
gb = gb,
cog_a = cog_a,
cog_b = cog_b,
a_samples = a_samples,
b_samples = b_samples,
highlyvariable = FALSE,
cooc_p = 0.05,
cooc_h = c(0.70,0.95),
cooc_cor_method = "pearson",
a_universe = a_universe,
a_id2go = a_id2go,
common_evo_nodes = common_evo_nodes,
sep = ",\ "
)## [1] "Tidy up data"
## [1] "Merge data"
## [1] "Correlations"
## [1] "PCA"
## [1] "Co-Occurrence"
## [1] "Common genes in Correlations"
## The top five similar pair of stages are: 17.42948 17.57871 18.07031 18.2674 18.41027
## [1] "Subsetting count matrices"
## [1] "Model fitting and top genes"
## [1] "Common genes in Correlations (GO)"
## [1] "Starting analysis 1 of 5"##
## Building most specific GOs .....## ( 15540 GO terms found. )##
## Build GO DAG topology ..........## ( 15584 GO terms and 36018 relations. )##
## Annotating nodes ...............## ( 15464 genes annotated to the GO terms. )##
## -- Classic Algorithm --
##
## the algorithm is scoring 216 nontrivial nodes
## parameters:
## test statistic: fisher## [1] "Starting analysis 2 of 5"##
## Building most specific GOs .....## ( 15540 GO terms found. )##
## Build GO DAG topology ..........## ( 15584 GO terms and 36018 relations. )##
## Annotating nodes ...............## ( 15464 genes annotated to the GO terms. )##
## -- Classic Algorithm --
##
## the algorithm is scoring 217 nontrivial nodes
## parameters:
## test statistic: fisher## [1] "Starting analysis 3 of 5"##
## Building most specific GOs .....## ( 15540 GO terms found. )##
## Build GO DAG topology ..........## ( 15584 GO terms and 36018 relations. )##
## Annotating nodes ...............## ( 15464 genes annotated to the GO terms. )##
## -- Classic Algorithm --
##
## the algorithm is scoring 582 nontrivial nodes
## parameters:
## test statistic: fisher## [1] "Starting analysis 4 of 5"##
## Building most specific GOs .....## ( 15540 GO terms found. )##
## Build GO DAG topology ..........## ( 15584 GO terms and 36018 relations. )##
## Annotating nodes ...............## ( 15464 genes annotated to the GO terms. )##
## -- Classic Algorithm --
##
## the algorithm is scoring 204 nontrivial nodes
## parameters:
## test statistic: fisher## [1] "Starting analysis 5 of 5"##
## Building most specific GOs .....## ( 15540 GO terms found. )##
## Build GO DAG topology ..........## ( 15584 GO terms and 36018 relations. )##
## Annotating nodes ...............## ( 15464 genes annotated to the GO terms. )##
## -- Classic Algorithm --
##
## the algorithm is scoring 441 nontrivial nodes
## parameters:
## test statistic: fisher## [1] "Common genes in Correlations (age)"
## [1] "Pairwise Orthology Overlap Strategy across modules -- hypergeometric and binonmial tests"
## [1] "Getting info on genes from shared families across modules"
## [1] "Starting analysis 1 of 20"##
## Building most specific GOs .....## ( 15540 GO terms found. )##
## Build GO DAG topology ..........## ( 15584 GO terms and 36018 relations. )##
## Annotating nodes ...............## ( 15464 genes annotated to the GO terms. )##
## -- Classic Algorithm --
##
## the algorithm is scoring 1828 nontrivial nodes
## parameters:
## test statistic: fisher## [1] "Starting analysis 2 of 20"##
## Building most specific GOs .....## ( 15540 GO terms found. )##
## Build GO DAG topology ..........## ( 15584 GO terms and 36018 relations. )##
## Annotating nodes ...............## ( 15464 genes annotated to the GO terms. )##
## -- Classic Algorithm --
##
## the algorithm is scoring 1653 nontrivial nodes
## parameters:
## test statistic: fisher## [1] "Starting analysis 3 of 20"##
## Building most specific GOs .....## ( 15540 GO terms found. )##
## Build GO DAG topology ..........## ( 15584 GO terms and 36018 relations. )##
## Annotating nodes ...............## ( 15464 genes annotated to the GO terms. )##
## -- Classic Algorithm --
##
## the algorithm is scoring 2000 nontrivial nodes
## parameters:
## test statistic: fisher## [1] "Starting analysis 4 of 20"##
## Building most specific GOs .....## ( 15540 GO terms found. )##
## Build GO DAG topology ..........## ( 15584 GO terms and 36018 relations. )##
## Annotating nodes ...............## ( 15464 genes annotated to the GO terms. )##
## -- Classic Algorithm --
##
## the algorithm is scoring 2471 nontrivial nodes
## parameters:
## test statistic: fisher## [1] "Starting analysis 5 of 20"##
## Building most specific GOs .....## ( 15540 GO terms found. )##
## Build GO DAG topology ..........## ( 15584 GO terms and 36018 relations. )##
## Annotating nodes ...............## ( 15464 genes annotated to the GO terms. )##
## -- Classic Algorithm --
##
## the algorithm is scoring 778 nontrivial nodes
## parameters:
## test statistic: fisher## [1] "Starting analysis 6 of 20"##
## Building most specific GOs .....## ( 15540 GO terms found. )##
## Build GO DAG topology ..........## ( 15584 GO terms and 36018 relations. )##
## Annotating nodes ...............## ( 15464 genes annotated to the GO terms. )##
## -- Classic Algorithm --
##
## the algorithm is scoring 3558 nontrivial nodes
## parameters:
## test statistic: fisher## [1] "Starting analysis 7 of 20"##
## Building most specific GOs .....## ( 15540 GO terms found. )##
## Build GO DAG topology ..........## ( 15584 GO terms and 36018 relations. )##
## Annotating nodes ...............## ( 15464 genes annotated to the GO terms. )##
## -- Classic Algorithm --
##
## the algorithm is scoring 1329 nontrivial nodes
## parameters:
## test statistic: fisher## [1] "Starting analysis 8 of 20"##
## Building most specific GOs .....## ( 15540 GO terms found. )##
## Build GO DAG topology ..........## ( 15584 GO terms and 36018 relations. )##
## Annotating nodes ...............## ( 15464 genes annotated to the GO terms. )##
## -- Classic Algorithm --
##
## the algorithm is scoring 2967 nontrivial nodes
## parameters:
## test statistic: fisher## [1] "Starting analysis 9 of 20"##
## Building most specific GOs .....## ( 15540 GO terms found. )##
## Build GO DAG topology ..........## ( 15584 GO terms and 36018 relations. )##
## Annotating nodes ...............## ( 15464 genes annotated to the GO terms. )##
## -- Classic Algorithm --
##
## the algorithm is scoring 1276 nontrivial nodes
## parameters:
## test statistic: fisher## [1] "Starting analysis 10 of 20"##
## Building most specific GOs .....## ( 15540 GO terms found. )##
## Build GO DAG topology ..........## ( 15584 GO terms and 36018 relations. )##
## Annotating nodes ...............## ( 15464 genes annotated to the GO terms. )##
## -- Classic Algorithm --
##
## the algorithm is scoring 1126 nontrivial nodes
## parameters:
## test statistic: fisher## [1] "Starting analysis 11 of 20"##
## Building most specific GOs .....## ( 15540 GO terms found. )##
## Build GO DAG topology ..........## ( 15584 GO terms and 36018 relations. )##
## Annotating nodes ...............## ( 15464 genes annotated to the GO terms. )##
## -- Classic Algorithm --
##
## the algorithm is scoring 2020 nontrivial nodes
## parameters:
## test statistic: fisher## [1] "Starting analysis 12 of 20"##
## Building most specific GOs .....## ( 15540 GO terms found. )##
## Build GO DAG topology ..........## ( 15584 GO terms and 36018 relations. )##
## Annotating nodes ...............## ( 15464 genes annotated to the GO terms. )##
## -- Classic Algorithm --
##
## the algorithm is scoring 1953 nontrivial nodes
## parameters:
## test statistic: fisher## [1] "Starting analysis 13 of 20"##
## Building most specific GOs .....## ( 15540 GO terms found. )##
## Build GO DAG topology ..........## ( 15584 GO terms and 36018 relations. )##
## Annotating nodes ...............## ( 15464 genes annotated to the GO terms. )##
## -- Classic Algorithm --
##
## the algorithm is scoring 2969 nontrivial nodes
## parameters:
## test statistic: fisher## [1] "Starting analysis 14 of 20"##
## Building most specific GOs .....## ( 15540 GO terms found. )##
## Build GO DAG topology ..........## ( 15584 GO terms and 36018 relations. )##
## Annotating nodes ...............## ( 15464 genes annotated to the GO terms. )##
## -- Classic Algorithm --
##
## the algorithm is scoring 1433 nontrivial nodes
## parameters:
## test statistic: fisher## [1] "Starting analysis 15 of 20"##
## Building most specific GOs .....## ( 15540 GO terms found. )##
## Build GO DAG topology ..........## ( 15584 GO terms and 36018 relations. )##
## Annotating nodes ...............## ( 15464 genes annotated to the GO terms. )##
## -- Classic Algorithm --
##
## the algorithm is scoring 371 nontrivial nodes
## parameters:
## test statistic: fisher## [1] "Starting analysis 16 of 20"##
## Building most specific GOs .....## ( 15540 GO terms found. )##
## Build GO DAG topology ..........## ( 15584 GO terms and 36018 relations. )##
## Annotating nodes ...............## ( 15464 genes annotated to the GO terms. )##
## -- Classic Algorithm --
##
## the algorithm is scoring 1533 nontrivial nodes
## parameters:
## test statistic: fisher## [1] "Starting analysis 17 of 20"##
## Building most specific GOs .....## ( 15540 GO terms found. )##
## Build GO DAG topology ..........## ( 15584 GO terms and 36018 relations. )##
## Annotating nodes ...............## ( 15464 genes annotated to the GO terms. )##
## -- Classic Algorithm --
##
## the algorithm is scoring 1427 nontrivial nodes
## parameters:
## test statistic: fisher## [1] "Starting analysis 18 of 20"##
## Building most specific GOs .....## ( 15540 GO terms found. )##
## Build GO DAG topology ..........## ( 15584 GO terms and 36018 relations. )##
## Annotating nodes ...............## ( 15464 genes annotated to the GO terms. )##
## -- Classic Algorithm --
##
## the algorithm is scoring 1045 nontrivial nodes
## parameters:
## test statistic: fisher## [1] "Starting analysis 19 of 20"##
## Building most specific GOs .....## ( 15540 GO terms found. )##
## Build GO DAG topology ..........## ( 15584 GO terms and 36018 relations. )##
## Annotating nodes ...............## ( 15464 genes annotated to the GO terms. )##
## -- Classic Algorithm --
##
## the algorithm is scoring 1362 nontrivial nodes
## parameters:
## test statistic: fisher## [1] "Starting analysis 20 of 20"##
## Building most specific GOs .....## ( 15540 GO terms found. )##
## Build GO DAG topology ..........## ( 15584 GO terms and 36018 relations. )##
## Annotating nodes ...............## ( 15464 genes annotated to the GO terms. )##
## -- Classic Algorithm --
##
## the algorithm is scoring 2292 nontrivial nodes
## parameters:
## test statistic: fisher## [1] "Plots"
## [1] "Generating results"
The heatmaps of pairwise correlations and jensen-shannon divergence between Ptychodera and Sea Urchin:
plot_cors(PFLA_SPUR_COMPARISON_OF$pairwise_correlations)
A quick sanity check that this is consistently observed regardles of genes by doing some bootstraping and checkin the mean observed JSD:
js_mean <- jsd_with_subsampling(
a_o = PFLA_SPUR_COMPARISON_OF$merged_data$a_o ,
b_o = PFLA_SPUR_COMPARISON_OF$merged_data$b_o,
n = 1000, p = 0.25
)
#relativise to min and max values
js_mean_rel <- relativise(js_mean$mean)
h3_avg_rel <- Heatmap(js_mean_rel,cluster_rows = F, cluster_columns = F, show_row_names = TRUE, name = "JSD", col = brewer.pal(10,"RdBu"))
draw(h3_avg_rel)
Here is the average expression profile of genes more expressed in the most similar stages between Ptychodera an Sea Urchin, including GO terms and age enrichment:
# Common genes, highly correlated
p1 <- plot_hicor_genes(
scatter = PFLA_SPUR_COMPARISON_OF$high_corr_genes$pairwise_data$hicor_topgenes[[1]]$plot_topgenes,
GO_table = PFLA_SPUR_COMPARISON_OF$high_corr_genes$GOs$GOtable[[1]],
age_table = PFLA_SPUR_COMPARISON_OF$high_corr_genes$age[[1]]$AgeperModule,
age_enrichemnt_hm = PFLA_SPUR_COMPARISON_OF$high_corr_genes$age[[1]]$heatmap
)
p2 <- plot_hicor_genes(
scatter = PFLA_SPUR_COMPARISON_OF$high_corr_genes$pairwise_data$hicor_topgenes[[2]]$plot_topgenes,
GO_table = PFLA_SPUR_COMPARISON_OF$high_corr_genes$GOs$GOtable[[2]],
age_table = PFLA_SPUR_COMPARISON_OF$high_corr_genes$age[[2]]$AgeperModule,
age_enrichemnt_hm = PFLA_SPUR_COMPARISON_OF$high_corr_genes$age[[2]]$heatmap
)
p3 <- plot_hicor_genes(
scatter = PFLA_SPUR_COMPARISON_OF$high_corr_genes$pairwise_data$hicor_topgenes[[3]]$plot_topgenes,
GO_table = PFLA_SPUR_COMPARISON_OF$high_corr_genes$GOs$GOtable[[3]],
age_table = PFLA_SPUR_COMPARISON_OF$high_corr_genes$age[[3]]$AgeperModule,
age_enrichemnt_hm = PFLA_SPUR_COMPARISON_OF$high_corr_genes$age[[3]]$heatmap
)
plot_grid(p1,p2,p3,ncol = 1)
And the co-ocurrence matrix shocasing ptychodera gastrulation is similar to sea urchin gastrulation as well as similarities between larval stages
Heatmap(
name = "co-occurrence",
PFLA_SPUR_COMPARISON_OF$coocurrence_analysis$cooccurrence[1:16,17:33],
cluster_rows = F,
cluster_columns = F,
col = sequential_hcl(10,"YlOrRd", rev = TRUE)
)
Below the orthology overlap strategy showcasing similarities at the gene family usage between species:
pf_avg_hm+
PFLA_SPUR_COMPARISON_OF$plots$orthology_overlap_binomial_hm+
PFLA_SPUR_COMPARISON_OF$plots$orthology_overlap_hypgeom_hm
An overview of the stats of these tests:
head(PFLA_SPUR_COMPARISON_OF$orthology_overlap_modules$pairwise_module_comparison$stats)## module_a module_b success_in_samples sample_size success_in_pop gene_POP
## 2 01 1 31 1336 234 15568
## 3 01 2 10 1336 148 15568
## 4 01 3 4 1336 64 15568
## 5 01 4 11 1336 146 15568
## 6 01 5 17 1336 145 15568
## 7 01 6 8 1336 71 15568
## hypgeom_pval hypgeom_log binom_pval
## 2 0.01009057 4.5961540 0.02345195
## 3 0.82667662 0.1903417 0.57116633
## 4 0.81058437 0.2099999 0.67082907
## 5 0.71768583 0.3317234 0.77691380
## 6 0.11610079 2.1532966 0.19703270
## 7 0.26268361 1.3368050 0.41112537
## gfams_common
## 2 OG0000250, OG0000045, OG0000082, OG0004184, OG0004407, OG0000312, OG0004082, OG0000020, OG0007691, OG0000016, OG0001670, OG0000972, OG0002787, OG0007497, OG0000213, OG0005448, OG0004514, OG0008797, OG0000115, OG0003480, OG0004527, OG0008389, OG0008350, OG0012844, OG0004883, OG0000623, OG0000159, OG0006243, OG0010209, OG0000360, OG0004621
## 3 OG0000230, OG0000116, OG0000312, OG0000310, OG0000019, OG0001412, OG0000236, OG0008389, OG0000159, OG0001614
## 4 OG0000152, OG0003047, OG0007478, OG0001232
## 5 OG0000219, OG0000685, OG0000879, OG0000352, OG0000016, OG0000019, OG0000006, OG0001719, OG0000056, OG0000777, OG0000297
## 6 OG0000054, OG0000082, OG0001903, OG0000014, OG0003365, OG0000588, OG0000067, OG0000152, OG0000218, OG0005750, OG0000007, OG0000019, OG0000011, OG0002083, OG0000056, OG0000083, OG0000456
## 7 OG0001278, OG0000016, OG0000066, OG0000130, OG0000152, OG0000681, OG0000011, OG0000213
## gfams_excl_a
## 2 OG0004246, OG0021513, OG0002904, OG0033168, OG0014398, OG0006163, OG0008719, OG0019224, OG0014404, OG0001209, OG0000910, OG0001311, OG0000025, OG0006944, OG0000145, OG0000505, OG0001374, OG0000219, OG0003064, OG0008829, OG0011330, OG0015711, OG0011176, OG0004787, OG0001767, OG0008460, OG0002345, OG0001382, OG0001890, OG0000685, OG0000054, OG0033196, OG0001523, OG0005198, OG0000418, OG0002138, OG0006081, OG0002353, OG0014247, OG0009823, OG0002541, OG0001459, OG0047540, OG0000349, OG0009199, OG0002052, OG0015302, OG0005120, OG0002493, OG0001333, OG0000230, OG0011983, OG0002088, OG0002358, OG0010240, OG0008657, OG0005197, OG0003381, OG0010846, OG0001602, OG0000437, OG0006899, OG0009632, OG0001160, OG0000825, OG0002810, OG0002347, OG0004869, OG0005298, OG0003677, OG0009309, OG0002019, OG0003790, OG0009183, OG0001246, OG0000174, OG0003013, OG0048110, OG0048111, OG0047319, OG0000879, OG0007188, OG0003847, OG0010448, OG0000352, OG0006974, OG0002646, OG0008414, OG0001127, OG0006264, OG0000534, OG0001181, OG0001082, OG0000575, OG0006609, OG0003772, OG0026859, OG0033389, OG0006615, OG0006232, OG0008054, OG0007774, OG0000116, OG0011706, OG0048097, OG0008815, OG0007339, OG0000119, OG0007647, OG0033387, OG0004377, OG0009082, OG0003543, OG0009102, OG0004060, OG0004521, OG0001608, OG0004226, OG0007300, OG0017602, OG0011272, OG0000077, OG0015159, OG0005372, OG0003676, OG0004786, OG0003699, OG0004144, OG0006545, OG0001316, OG0010641, OG0000048, OG0001419, OG0003900, OG0001559, OG0006125, OG0004041, OG0001279, OG0008602, OG0003998, OG0001741, OG0005614, OG0047286, OG0001188, OG0008569, OG0005822, OG0009620, OG0000239, OG0005465, OG0006172, OG0047260, OG0005633, OG0004692, OG0007653, OG0005613, OG0002487, OG0010542, OG0008027, OG0001903, OG0007461, OG0002639, OG0009150, OG0002368, OG0003360, OG0014112, OG0004751, OG0006353, OG0002028, OG0017239, OG0008422, OG0000257, OG0009058, OG0000363, OG0006385, OG0026085, OG0009475, OG0003471, OG0002875, OG0000014, OG0004884, OG0003435, OG0006849, OG0005055, OG0006149, OG0010827, OG0000724, OG0005079, OG0000128, OG0005270, OG0000391, OG0004907, OG0009724, OG0005699, OG0000696, OG0008567, OG0004202, OG0008673, OG0007817, OG0002761, OG0000102, OG0007314, OG0007541, OG0002376, OG0005124, OG0001018, OG0002722, OG0000548, OG0003135, OG0006225, OG0006001, OG0004644, OG0020520, OG0003004, OG0002975, OG0001673, OG0000124, OG0002103, OG0007177, OG0006747, OG0007277, OG0008649, OG0009723, OG0013721, OG0047278, OG0007309, OG0018375, OG0006373, OG0005175, OG0008311, OG0010226, OG0011256, OG0007335, OG0007450, OG0003292, OG0001490, OG0012810, OG0006136, OG0001177, OG0000429, OG0004645, OG0012872, OG0003625, OG0003859, OG0008028, OG0008767, OG0013481, OG0000121, OG0005439, OG0033134, OG0010155, OG0008658, OG0000288, OG0047398, OG0007304, OG0003340, OG0005283, OG0004209, OG0002414, OG0047402, OG0004014, OG0000994, OG0004228, OG0001103, OG0006259, OG0018867, OG0001463, OG0009125, OG0021810, OG0003365, OG0009396, OG0000279, OG0008580, OG0000185, OG0017301, OG0018987, OG0047412, OG0000037, OG0002267, OG0003855, OG0008809, OG0000676, OG0004757, OG0002835, OG0001278, OG0003370, OG0010568, OG0000325, OG0007080, OG0000175, OG0001199, OG0000527, OG0004572, OG0003679, OG0003309, OG0001344, OG0008651, OG0047742, OG0001439, OG0000214, OG0033031, OG0011442, OG0010154, OG0019411, OG0001722, OG0010333, OG0006702, OG0020483, OG0003670, OG0005445, OG0008682, OG0019322, OG0009216, OG0002410, OG0002740, OG0003536, OG0005512, OG0026163, OG0007183, OG0047882, OG0033329, OG0000341, OG0018008, OG0015529, OG0013558, OG0008510, OG0003885, OG0005950, OG0007317, OG0001857, OG0004731, OG0001864, OG0000317, OG0006379, OG0005677, OG0000434, OG0005045, OG0004942, OG0012249, OG0000247, OG0001016, OG0005855, OG0001159, OG0000293, OG0001211, OG0010890, OG0047706, OG0001753, OG0033238, OG0000031, OG0010107, OG0047708, OG0047709, OG0008009, OG0004375, OG0018953, OG0014657, OG0000567, OG0003158, OG0047115, OG0006138, OG0007042, OG0005590, OG0007827, OG0007496, OG0001661, OG0003284, OG0000523, OG0008305, OG0026053, OG0004616, OG0000390, OG0011708, OG0004622, OG0001656, OG0001328, OG0002912, OG0001400, OG0047159, OG0001633, OG0007495, OG0018083, OG0001551, OG0003005, OG0003702, OG0005150, OG0007667, OG0009675, OG0002531, OG0007981, OG0005173, OG0002555, OG0006338, OG0009164, OG0006572, OG0005837, OG0006199, OG0047280, OG0002435, OG0007968, OG0000477, OG0010610, OG0000780, OG0007392, OG0000113, OG0006003, OG0001252, OG0001730, OG0033167, OG0003507, OG0008760, OG0002193, OG0001235, OG0005900, OG0001625, OG0005506, OG0019476, OG0033376, OG0026262, OG0019029, OG0000810, OG0009335, OG0004160, OG0007119, OG0000109, OG0005144, OG0009421, OG0000925, OG0008519, OG0005294, OG0001986, OG0048045, OG0026170, OG0000392, OG0002164, OG0015720, OG0000732, OG0006106, OG0009092, OG0033373, OG0006316, OG0016075, OG0007793, OG0011977, OG0004860, OG0048067, OG0008872, OG0000066, OG0002754, OG0005191, OG0000111, OG0010545, OG0006341, OG0048068, OG0007795, OG0005277, OG0006876, OG0007847, OG0007509, OG0004389, OG0009293, OG0002789, OG0013604, OG0000588, OG0002502, OG0001337, OG0000067, OG0002562, OG0000130, OG0021764, OG0000152, OG0047671, OG0002224, OG0001676, OG0017364, OG0009816, OG0002212, OG0008876, OG0001858, OG0012114, OG0006990, OG0001087, OG0000400, OG0015679, OG0047676, OG0047677, OG0047678, OG0015693, OG0001360, OG0010193, OG0000727, OG0002359, OG0004296, OG0002196, OG0001493, OG0007075, OG0003388, OG0003813, OG0033230, OG0002655, OG0000316, OG0001936, OG0000218, OG0003383, OG0009424, OG0007220, OG0003492, OG0001820, OG0006276, OG0003355, OG0000063, OG0000034, OG0009824, OG0021840, OG0010181, OG0000188, OG0009152, OG0002153, OG0003176, OG0008857, OG0004921, OG0007661, OG0005766, OG0009135, OG0005142, OG0005895, OG0000326, OG0006745, OG0003716, OG0000313, OG0007338, OG0002948, OG0005242, OG0001404, OG0006683, OG0003925, OG0015535, OG0013496, OG0005418, OG0011721, OG0002779, OG0008547, OG0008079, OG0000953, OG0026255, OG0007122, OG0004121, OG0002522, OG0013239, OG0025898, OG0018068, OG0002058, OG0007806, OG0000572, OG0011766, OG0001960, OG0003926, OG0011111, OG0002418, OG0006983, OG0004808, OG0001869, OG0011654, OG0000894, OG0000681, OG0001410, OG0005750, OG0000997, OG0002712, OG0018973, OG0000337, OG0002537, OG0012840, OG0006537, OG0019357, OG0018162, OG0001233, OG0001287, OG0004402, OG0008328, OG0001704, OG0007006, OG0007044, OG0021812, OG0008075, OG0010104, OG0021772, OG0011698, OG0011672, OG0000310, OG0016436, OG0000007, OG0001871, OG0002401, OG0008977, OG0000322, OG0007452, OG0047857, OG0005570, OG0006668, OG0000019, OG0047860, OG0031954, OG0004707, OG0006365, OG0004587, OG0006881, OG0007298, OG0008432, OG0002622, OG0002095, OG0009165, OG0005423, OG0003157, OG0007800, OG0026226, OG0001519, OG0003294, OG0013488, OG0033314, OG0007010, OG0004697, OG0011168, OG0003953, OG0004781, OG0007628, OG0008043, OG0047849, OG0019213, OG0003011, OG0017282, OG0001186, OG0005609, OG0007929, OG0006262, OG0005135, OG0047448, OG0004482, OG0005245, OG0001396, OG0012225, OG0001095, OG0000895, OG0001956, OG0047453, OG0000406, OG0010920, OG0005235, OG0003884, OG0004471, OG0005272, OG0002452, OG0016427, OG0003481, OG0010861, OG0000848, OG0005979, OG0000006, OG0003134, OG0004758, OG0000142, OG0002517, OG0002149, OG0005413, OG0015531, OG0005412, OG0007633, OG0005383, OG0004628, OG0006165, OG0007527, OG0002491, OG0001666, OG0004403, OG0001678, OG0002336, OG0000619, OG0001790, OG0004627, OG0008280, OG0011759, OG0001584, OG0047486, OG0000011, OG0007089, OG0001555, OG0001747, OG0047488, OG0002725, OG0002459, OG0008866, OG0005373, OG0005007, OG0000084, OG0000948, OG0007773, OG0008257, OG0001139, OG0002998, OG0006993, OG0003047, OG0004803, OG0002403, OG0001511, OG0001719, OG0000731, OG0000364, OG0008323, OG0005795, OG0007400, OG0000256, OG0003054, OG0047520, OG0047499, OG0001401, OG0007070, OG0023006, OG0005714, OG0001001, OG0006094, OG0009043, OG0006256, OG0026187, OG0001496, OG0033243, OG0047087, OG0005103, OG0006389, OG0000137, OG0010223, OG0014825, OG0002293, OG0000209, OG0002841, OG0005040, OG0012719, OG0000090, OG0000716, OG0012716, OG0002063, OG0013613, OG0026025, OG0006387, OG0007672, OG0000040, OG0001668, OG0014127, OG0003189, OG0002629, OG0006960, OG0002879, OG0000638, OG0004820, OG0007399, OG0002499, OG0001780, OG0000074, OG0033032, OG0002135, OG0001170, OG0008595, OG0008275, OG0001554, OG0002685, OG0006694, OG0011208, OG0007284, OG0003549, OG0047110, OG0007848, OG0000890, OG0000909, OG0009839, OG0047086, OG0011032, OG0033127, OG0002022, OG0001306, OG0006686, OG0019509, OG0001412, OG0003376, OG0000254, OG0001613, OG0048026, OG0002656, OG0012829, OG0016442, OG0004935, OG0001743, OG0004710, OG0002070, OG0008317, OG0003557, OG0001314, OG0004345, OG0001802, OG0000453, OG0000064, OG0007660, OG0009093, OG0001305, OG0007155, OG0008318, OG0017006, OG0000529, OG0003210, OG0018984, OG0003868, OG0003036, OG0013770, OG0000412, OG0000236, OG0007329, OG0000151, OG0009658, OG0003737, OG0002318, OG0009568, OG0005974, OG0009129, OG0000574, OG0006376, OG0006570, OG0005522, OG0008947, OG0009168, OG0005919, OG0002628, OG0002751, OG0002217, OG0000592, OG0006987, OG0009182, OG0004909, OG0007239, OG0002855, OG0008276, OG0001571, OG0005201, OG0007417, OG0013029, OG0006908, OG0002508, OG0000201, OG0007820, OG0007316, OG0003872, OG0005857, OG0007487, OG0000323, OG0001582, OG0001220, OG0007736, OG0001914, OG0009544, OG0006308, OG0008524, OG0005488, OG0002799, OG0001451, OG0005377, OG0047192, OG0008284, OG0005816, OG0003128, OG0003387, OG0005815, OG0007478, OG0009565, OG0008411, OG0006634, OG0001525, OG0002479, OG0005915, OG0008870, OG0004776, OG0000721, OG0001799, OG0047174, OG0005321, OG0009411, OG0008074, OG0002297, OG0010956, OG0009571, OG0003639, OG0003291, OG0000344, OG0002344, OG0009582, OG0002211, OG0000943, OG0000643, OG0006604, OG0009180, OG0008837, OG0001164, OG0002728, OG0004988, OG0005535, OG0000800, OG0000168, OG0047166, OG0007775, OG0004789, OG0000847, OG0003927, OG0004788, OG0002166, OG0004995, OG0009195, OG0004914, OG0006096, OG0006010, OG0001720, OG0033075, OG0012827, OG0000241, OG0001216, OG0001234, OG0008046, OG0004903, OG0000402, OG0003371, OG0011266, OG0007243, OG0002354, OG0003251, OG0004216, OG0002908, OG0007324, OG0006513, OG0003769, OG0019446, OG0004460, OG0002034, OG0007253, OG0006180, OG0002590, OG0011764, OG0004312, OG0007111, OG0012858, OG0047245, OG0000612, OG0034480, OG0001487, OG0009140, OG0011207, OG0001405, OG0008496, OG0000546, OG0002461, OG0012180, OG0002421, OG0002716, OG0001868, OG0000235, OG0006098, OG0008861, OG0007160, OG0007745, OG0016997, OG0002668, OG0004872, OG0006118, OG0004842, OG0001572, OG0003048, OG0024547, OG0002201, OG0007114, OG0011718, OG0006100, OG0003574, OG0000978, OG0009485, OG0009587, OG0000359, OG0000273, OG0009091, OG0005524, OG0002083, OG0005898, OG0007424, OG0001953, OG0000781, OG0007836, OG0006212, OG0000056, OG0008598, OG0047335, OG0047313, OG0013674, OG0007048, OG0047339, OG0005651, OG0009896, OG0000818, OG0000777, OG0006678, OG0007601, OG0000311, OG0003567, OG0006566, OG0002902, OG0005701, OG0001883, OG0002724, OG0002433, OG0007977, OG0001776, OG0006070, OG0015528, OG0033109, OG0002234, OG0017003, OG0003356, OG0005395, OG0008588, OG0005831, OG0000297, OG0032781, OG0004405, OG0004125, OG0004504, OG0002602, OG0002090, OG0005208, OG0047325, OG0007019, OG0000046, OG0004252, OG0008577, OG0004738, OG0007479, OG0007801, OG0002286, OG0006642, OG0002228, OG0000083, OG0000431, OG0008254, OG0004340, OG0004605, OG0005134, OG0011055, OG0002888, OG0002666, OG0004559, OG0001452, OG0013656, OG0003330, OG0015166, OG0003854, OG0007457, OG0008252, OG0005752, OG0026896, OG0000573, OG0008227, OG0011694, OG0005583, OG0001136, OG0047622, OG0009935, OG0008250, OG0011241, OG0014001, OG0001614, OG0004270, OG0008302, OG0023995, OG0001030, OG0015805, OG0006770, OG0003115, OG0011774, OG0002042, OG0006015, OG0011635, OG0002246, OG0020445, OG0003239, OG0004477, OG0002295, OG0005824, OG0004960, OG0014681, OG0033045, OG0002853, OG0009611, OG0001768, OG0008875, OG0003820, OG0003470, OG0047628, OG0001031, OG0004311, OG0000679, OG0006302, OG0001503, OG0008016, OG0001728, OG0026199, OG0026211, OG0007176, OG0009633, OG0007058, OG0010251, OG0047818, OG0000183, OG0008581, OG0003390, OG0007097, OG0003266, OG0000242, OG0001194, OG0047794, OG0009580, OG0004944, OG0002490, OG0005429, OG0007808, OG0000858, OG0015744, OG0008394, OG0001495, OG0007928, OG0001632, OG0005475, OG0006342, OG0002750, OG0001079, OG0007469, OG0006297, OG0002756, OG0006322, OG0005937, OG0004349, OG0003666, OG0008094, OG0009872, OG0011294, OG0000774, OG0006550, OG0001270, OG0004547, OG0005600, OG0007516, OG0009314, OG0014584, OG0014695, OG0000536, OG0017189, OG0004244, OG0008864, OG0002715, OG0006933, OG0005086, OG0003265, OG0003441, OG0008011, OG0000861, OG0001247, OG0003447, OG0047966, OG0001852, OG0003514, OG0007909, OG0033353, OG0000664, OG0010130, OG0003842, OG0021853, OG0005659, OG0002155, OG0002973, OG0000097, OG0021836, OG0003358, OG0006997, OG0002989, OG0005786, OG0001356, OG0006676, OG0005568, OG0001369, OG0005599, OG0000415, OG0002986, OG0005772, OG0007610, OG0000790, OG0001663, OG0018205, OG0001232, OG0001317, OG0002209, OG0014490, OG0000456, OG0001643, OG0047723, OG0006053, OG0002545, OG0024464, OG0005625, OG0007170, OG0003611, OG0009430, OG0010930, OG0000601, OG0006363, OG0007778, OG0006167, OG0006982, OG0003320, OG0003368, OG0001378, OG0007823, OG0002565, OG0003554, OG0000850, OG0004343, OG0008381, OG0016407, OG0005955, OG0004459, OG0003948, OG0005195, OG0009666, OG0000282, OG0002235, OG0007732, OG0005603, OG0008301, OG0006890, OG0003177, OG0009157, OG0008222, OG0003548, OG0007649, OG0002312, OG0010914, OG0004245, OG0001606, OG0009174, OG0047591, OG0004362, OG0009584, OG0007347, OG0008644, OG0003147, OG0001798, OG0002084, OG0000298, OG0001756, OG0002321, OG0016513, OG0006119, OG0005119, OG0047607, OG0001510, OG0006679, OG0008698, OG0008320, OG0003863, OG0005836, OG0000662, OG0006919, OG0011702, OG0002285, OG0009813, OG0001627, OG0006250, OG0047614, OG0011692, OG0009451, OG0008364, OG0002737, OG0047745, OG0000586, OG0026193, OG0006614, OG0019017, OG0001361, OG0003778, OG0004824, OG0000135, OG0004294, OG0012241, OG0011367, OG0009112, OG0000300, OG0016416, OG0010518, OG0011596, OG0007074, OG0001023, OG0007612, OG0002994, OG0004746, OG0007219, OG0000872, OG0013589
## 3 OG0004246, OG0021513, OG0002904, OG0033168, OG0014398, OG0006163, OG0008719, OG0019224, OG0014404, OG0001209, OG0000910, OG0001311, OG0000025, OG0006944, OG0000145, OG0000505, OG0001374, OG0000219, OG0003064, OG0008829, OG0011330, OG0015711, OG0011176, OG0004787, OG0001767, OG0008460, OG0002345, OG0001382, OG0001890, OG0000685, OG0000054, OG0033196, OG0001523, OG0005198, OG0000418, OG0002138, OG0006081, OG0002353, OG0014247, OG0009823, OG0000250, OG0002541, OG0001459, OG0047540, OG0000349, OG0009199, OG0002052, OG0015302, OG0005120, OG0002493, OG0001333, OG0011983, OG0002088, OG0002358, OG0010240, OG0008657, OG0005197, OG0003381, OG0010846, OG0001602, OG0000437, OG0006899, OG0009632, OG0001160, OG0000825, OG0002810, OG0002347, OG0004869, OG0005298, OG0003677, OG0009309, OG0002019, OG0003790, OG0009183, OG0001246, OG0000174, OG0000045, OG0003013, OG0048110, OG0048111, OG0047319, OG0000879, OG0007188, OG0003847, OG0010448, OG0000352, OG0006974, OG0002646, OG0008414, OG0000082, OG0001127, OG0006264, OG0000534, OG0001181, OG0001082, OG0000575, OG0006609, OG0003772, OG0026859, OG0033389, OG0006615, OG0006232, OG0008054, OG0007774, OG0011706, OG0048097, OG0008815, OG0007339, OG0000119, OG0007647, OG0033387, OG0004377, OG0009082, OG0003543, OG0009102, OG0004060, OG0004521, OG0001608, OG0004226, OG0007300, OG0017602, OG0011272, OG0000077, OG0015159, OG0005372, OG0003676, OG0004786, OG0003699, OG0004144, OG0006545, OG0001316, OG0010641, OG0000048, OG0001419, OG0003900, OG0001559, OG0006125, OG0004041, OG0001279, OG0008602, OG0003998, OG0001741, OG0005614, OG0047286, OG0001188, OG0008569, OG0005822, OG0009620, OG0000239, OG0005465, OG0006172, OG0047260, OG0005633, OG0004692, OG0007653, OG0005613, OG0002487, OG0010542, OG0008027, OG0001903, OG0007461, OG0002639, OG0009150, OG0002368, OG0003360, OG0014112, OG0004751, OG0006353, OG0002028, OG0017239, OG0008422, OG0000257, OG0009058, OG0000363, OG0006385, OG0026085, OG0009475, OG0003471, OG0002875, OG0000014, OG0004884, OG0003435, OG0006849, OG0005055, OG0006149, OG0010827, OG0000724, OG0005079, OG0000128, OG0005270, OG0000391, OG0004907, OG0009724, OG0005699, OG0000696, OG0008567, OG0004202, OG0004184, OG0008673, OG0007817, OG0002761, OG0000102, OG0007314, OG0007541, OG0002376, OG0005124, OG0001018, OG0002722, OG0000548, OG0003135, OG0006225, OG0006001, OG0004644, OG0020520, OG0003004, OG0002975, OG0001673, OG0000124, OG0004407, OG0002103, OG0007177, OG0006747, OG0007277, OG0008649, OG0009723, OG0013721, OG0047278, OG0007309, OG0018375, OG0006373, OG0005175, OG0008311, OG0010226, OG0011256, OG0007335, OG0007450, OG0003292, OG0001490, OG0012810, OG0006136, OG0001177, OG0000429, OG0004645, OG0012872, OG0003625, OG0003859, OG0008028, OG0008767, OG0013481, OG0000121, OG0005439, OG0033134, OG0010155, OG0008658, OG0000288, OG0004082, OG0047398, OG0007304, OG0003340, OG0005283, OG0004209, OG0002414, OG0047402, OG0004014, OG0000994, OG0004228, OG0001103, OG0006259, OG0018867, OG0001463, OG0009125, OG0021810, OG0003365, OG0009396, OG0000279, OG0008580, OG0000185, OG0017301, OG0018987, OG0047412, OG0000037, OG0002267, OG0003855, OG0008809, OG0000676, OG0004757, OG0002835, OG0001278, OG0003370, OG0010568, OG0000325, OG0007080, OG0000175, OG0001199, OG0000527, OG0004572, OG0003679, OG0003309, OG0001344, OG0008651, OG0047742, OG0001439, OG0000214, OG0033031, OG0011442, OG0000020, OG0010154, OG0019411, OG0001722, OG0010333, OG0006702, OG0020483, OG0003670, OG0005445, OG0008682, OG0019322, OG0009216, OG0002410, OG0002740, OG0007691, OG0003536, OG0005512, OG0026163, OG0007183, OG0047882, OG0033329, OG0000341, OG0018008, OG0015529, OG0013558, OG0008510, OG0003885, OG0005950, OG0007317, OG0001857, OG0004731, OG0001864, OG0000317, OG0006379, OG0005677, OG0000434, OG0005045, OG0004942, OG0012249, OG0000247, OG0001016, OG0005855, OG0001159, OG0000293, OG0001211, OG0010890, OG0047706, OG0001753, OG0033238, OG0000031, OG0010107, OG0047708, OG0047709, OG0008009, OG0004375, OG0018953, OG0014657, OG0000567, OG0003158, OG0047115, OG0000016, OG0006138, OG0007042, OG0005590, OG0007827, OG0007496, OG0001661, OG0003284, OG0000523, OG0008305, OG0026053, OG0004616, OG0000390, OG0011708, OG0004622, OG0001656, OG0001328, OG0002912, OG0001400, OG0047159, OG0001633, OG0007495, OG0018083, OG0001551, OG0003005, OG0003702, OG0005150, OG0007667, OG0009675, OG0002531, OG0007981, OG0005173, OG0002555, OG0006338, OG0009164, OG0006572, OG0005837, OG0006199, OG0047280, OG0002435, OG0007968, OG0000477, OG0010610, OG0000780, OG0007392, OG0000113, OG0006003, OG0001252, OG0001730, OG0033167, OG0003507, OG0008760, OG0002193, OG0001235, OG0005900, OG0001625, OG0005506, OG0019476, OG0033376, OG0026262, OG0019029, OG0000810, OG0009335, OG0004160, OG0007119, OG0000109, OG0005144, OG0009421, OG0001670, OG0000925, OG0008519, OG0005294, OG0001986, OG0048045, OG0026170, OG0000392, OG0002164, OG0015720, OG0000732, OG0006106, OG0009092, OG0033373, OG0006316, OG0016075, OG0007793, OG0011977, OG0004860, OG0048067, OG0008872, OG0000066, OG0002754, OG0005191, OG0000111, OG0010545, OG0006341, OG0048068, OG0007795, OG0005277, OG0006876, OG0007847, OG0007509, OG0004389, OG0009293, OG0002789, OG0013604, OG0000588, OG0002502, OG0001337, OG0000067, OG0002562, OG0000130, OG0021764, OG0000152, OG0047671, OG0002224, OG0000972, OG0001676, OG0017364, OG0009816, OG0002212, OG0008876, OG0001858, OG0012114, OG0006990, OG0001087, OG0000400, OG0015679, OG0047676, OG0047677, OG0047678, OG0015693, OG0001360, OG0010193, OG0000727, OG0002359, OG0004296, OG0002196, OG0001493, OG0007075, OG0003388, OG0003813, OG0033230, OG0002655, OG0000316, OG0001936, OG0000218, OG0003383, OG0009424, OG0007220, OG0003492, OG0001820, OG0006276, OG0003355, OG0000063, OG0000034, OG0009824, OG0021840, OG0010181, OG0000188, OG0009152, OG0002153, OG0003176, OG0008857, OG0004921, OG0007661, OG0005766, OG0009135, OG0005142, OG0005895, OG0000326, OG0006745, OG0003716, OG0000313, OG0007338, OG0002948, OG0005242, OG0001404, OG0006683, OG0003925, OG0015535, OG0013496, OG0005418, OG0011721, OG0002779, OG0008547, OG0008079, OG0000953, OG0026255, OG0007122, OG0004121, OG0002522, OG0013239, OG0025898, OG0018068, OG0002058, OG0007806, OG0000572, OG0011766, OG0001960, OG0003926, OG0011111, OG0002418, OG0006983, OG0004808, OG0001869, OG0011654, OG0000894, OG0000681, OG0001410, OG0005750, OG0000997, OG0002712, OG0018973, OG0000337, OG0002537, OG0012840, OG0006537, OG0019357, OG0018162, OG0001233, OG0001287, OG0004402, OG0008328, OG0001704, OG0007006, OG0007044, OG0021812, OG0008075, OG0010104, OG0021772, OG0011698, OG0011672, OG0016436, OG0000007, OG0001871, OG0002401, OG0008977, OG0000322, OG0007452, OG0047857, OG0005570, OG0006668, OG0047860, OG0031954, OG0004707, OG0006365, OG0004587, OG0006881, OG0007298, OG0008432, OG0002622, OG0002095, OG0009165, OG0005423, OG0003157, OG0007800, OG0026226, OG0001519, OG0003294, OG0013488, OG0033314, OG0007010, OG0004697, OG0011168, OG0003953, OG0004781, OG0007628, OG0008043, OG0047849, OG0019213, OG0003011, OG0017282, OG0001186, OG0005609, OG0007929, OG0006262, OG0005135, OG0047448, OG0004482, OG0005245, OG0001396, OG0012225, OG0001095, OG0000895, OG0001956, OG0047453, OG0000406, OG0010920, OG0005235, OG0003884, OG0004471, OG0005272, OG0002452, OG0016427, OG0003481, OG0010861, OG0000848, OG0005979, OG0000006, OG0003134, OG0004758, OG0000142, OG0002517, OG0002149, OG0005413, OG0015531, OG0005412, OG0007633, OG0005383, OG0004628, OG0006165, OG0007527, OG0002787, OG0002491, OG0001666, OG0004403, OG0001678, OG0002336, OG0000619, OG0001790, OG0004627, OG0008280, OG0011759, OG0001584, OG0047486, OG0000011, OG0007089, OG0001555, OG0001747, OG0047488, OG0002725, OG0002459, OG0008866, OG0005373, OG0005007, OG0000084, OG0000948, OG0007773, OG0008257, OG0001139, OG0002998, OG0006993, OG0003047, OG0004803, OG0002403, OG0001511, OG0001719, OG0000731, OG0000364, OG0008323, OG0005795, OG0007497, OG0007400, OG0000256, OG0003054, OG0047520, OG0047499, OG0001401, OG0007070, OG0023006, OG0005714, OG0001001, OG0006094, OG0009043, OG0006256, OG0026187, OG0000213, OG0001496, OG0033243, OG0047087, OG0005103, OG0006389, OG0000137, OG0010223, OG0014825, OG0002293, OG0000209, OG0002841, OG0005040, OG0012719, OG0000090, OG0005448, OG0000716, OG0012716, OG0002063, OG0013613, OG0026025, OG0006387, OG0007672, OG0000040, OG0001668, OG0014127, OG0003189, OG0002629, OG0006960, OG0002879, OG0000638, OG0004820, OG0007399, OG0002499, OG0001780, OG0000074, OG0033032, OG0002135, OG0001170, OG0008595, OG0008275, OG0001554, OG0002685, OG0006694, OG0011208, OG0007284, OG0003549, OG0047110, OG0007848, OG0000890, OG0000909, OG0009839, OG0047086, OG0011032, OG0033127, OG0002022, OG0001306, OG0006686, OG0019509, OG0003376, OG0000254, OG0001613, OG0048026, OG0002656, OG0012829, OG0016442, OG0004935, OG0001743, OG0004710, OG0002070, OG0008317, OG0003557, OG0001314, OG0004345, OG0001802, OG0000453, OG0000064, OG0007660, OG0009093, OG0001305, OG0007155, OG0008318, OG0017006, OG0000529, OG0003210, OG0018984, OG0003868, OG0003036, OG0013770, OG0000412, OG0007329, OG0000151, OG0009658, OG0003737, OG0002318, OG0009568, OG0005974, OG0009129, OG0000574, OG0006376, OG0006570, OG0005522, OG0008947, OG0009168, OG0005919, OG0002628, OG0002751, OG0002217, OG0000592, OG0006987, OG0009182, OG0004909, OG0007239, OG0002855, OG0008276, OG0001571, OG0005201, OG0007417, OG0013029, OG0006908, OG0002508, OG0000201, OG0007820, OG0007316, OG0003872, OG0005857, OG0007487, OG0000323, OG0004514, OG0001582, OG0001220, OG0007736, OG0001914, OG0009544, OG0006308, OG0008524, OG0005488, OG0002799, OG0001451, OG0005377, OG0047192, OG0008284, OG0005816, OG0003128, OG0003387, OG0005815, OG0007478, OG0009565, OG0008411, OG0006634, OG0001525, OG0002479, OG0005915, OG0008870, OG0004776, OG0000721, OG0001799, OG0047174, OG0005321, OG0009411, OG0008074, OG0002297, OG0010956, OG0009571, OG0003639, OG0003291, OG0000344, OG0002344, OG0009582, OG0002211, OG0000943, OG0000643, OG0006604, OG0009180, OG0008837, OG0001164, OG0002728, OG0004988, OG0005535, OG0000800, OG0000168, OG0047166, OG0007775, OG0004789, OG0000847, OG0008797, OG0003927, OG0004788, OG0002166, OG0004995, OG0009195, OG0004914, OG0006096, OG0006010, OG0001720, OG0033075, OG0012827, OG0000241, OG0001216, OG0001234, OG0008046, OG0004903, OG0000402, OG0003371, OG0011266, OG0007243, OG0002354, OG0003251, OG0004216, OG0002908, OG0007324, OG0006513, OG0003769, OG0019446, OG0004460, OG0002034, OG0007253, OG0006180, OG0002590, OG0011764, OG0004312, OG0007111, OG0012858, OG0047245, OG0000612, OG0034480, OG0001487, OG0009140, OG0000115, OG0011207, OG0001405, OG0008496, OG0000546, OG0002461, OG0012180, OG0002421, OG0002716, OG0001868, OG0000235, OG0006098, OG0008861, OG0007160, OG0007745, OG0003480, OG0016997, OG0002668, OG0004872, OG0006118, OG0004842, OG0001572, OG0003048, OG0024547, OG0002201, OG0007114, OG0011718, OG0006100, OG0003574, OG0000978, OG0009485, OG0009587, OG0000359, OG0000273, OG0009091, OG0005524, OG0002083, OG0005898, OG0007424, OG0004527, OG0001953, OG0000781, OG0007836, OG0006212, OG0000056, OG0008598, OG0047335, OG0047313, OG0013674, OG0007048, OG0047339, OG0005651, OG0009896, OG0000818, OG0000777, OG0006678, OG0007601, OG0000311, OG0003567, OG0006566, OG0002902, OG0005701, OG0001883, OG0008350, OG0002724, OG0002433, OG0007977, OG0001776, OG0006070, OG0015528, OG0033109, OG0002234, OG0017003, OG0003356, OG0005395, OG0008588, OG0005831, OG0000297, OG0032781, OG0004405, OG0004125, OG0004504, OG0002602, OG0012844, OG0002090, OG0005208, OG0047325, OG0007019, OG0000046, OG0004252, OG0008577, OG0004738, OG0007479, OG0004883, OG0007801, OG0002286, OG0006642, OG0000623, OG0002228, OG0000083, OG0000431, OG0008254, OG0004340, OG0004605, OG0005134, OG0011055, OG0002888, OG0002666, OG0004559, OG0001452, OG0013656, OG0003330, OG0006243, OG0015166, OG0003854, OG0007457, OG0008252, OG0005752, OG0026896, OG0000573, OG0010209, OG0008227, OG0011694, OG0005583, OG0001136, OG0047622, OG0009935, OG0008250, OG0011241, OG0014001, OG0004270, OG0008302, OG0023995, OG0001030, OG0015805, OG0006770, OG0003115, OG0011774, OG0002042, OG0006015, OG0011635, OG0002246, OG0020445, OG0003239, OG0004477, OG0002295, OG0005824, OG0004960, OG0014681, OG0033045, OG0002853, OG0009611, OG0001768, OG0008875, OG0003820, OG0003470, OG0047628, OG0001031, OG0004311, OG0000679, OG0006302, OG0001503, OG0008016, OG0001728, OG0026199, OG0026211, OG0007176, OG0009633, OG0007058, OG0010251, OG0047818, OG0000183, OG0008581, OG0003390, OG0007097, OG0003266, OG0000242, OG0001194, OG0047794, OG0009580, OG0004944, OG0002490, OG0005429, OG0007808, OG0000858, OG0015744, OG0008394, OG0001495, OG0007928, OG0001632, OG0005475, OG0006342, OG0002750, OG0001079, OG0007469, OG0006297, OG0002756, OG0006322, OG0005937, OG0004349, OG0003666, OG0008094, OG0009872, OG0011294, OG0000774, OG0000360, OG0006550, OG0001270, OG0004547, OG0005600, OG0007516, OG0009314, OG0014584, OG0014695, OG0000536, OG0017189, OG0004244, OG0008864, OG0002715, OG0006933, OG0005086, OG0003265, OG0003441, OG0008011, OG0000861, OG0001247, OG0003447, OG0047966, OG0001852, OG0003514, OG0007909, OG0033353, OG0000664, OG0010130, OG0003842, OG0021853, OG0004621, OG0005659, OG0002155, OG0002973, OG0000097, OG0021836, OG0003358, OG0006997, OG0002989, OG0005786, OG0001356, OG0006676, OG0005568, OG0001369, OG0005599, OG0000415, OG0002986, OG0005772, OG0007610, OG0000790, OG0001663, OG0018205, OG0001232, OG0001317, OG0002209, OG0014490, OG0000456, OG0001643, OG0047723, OG0006053, OG0002545, OG0024464, OG0005625, OG0007170, OG0003611, OG0009430, OG0010930, OG0000601, OG0006363, OG0007778, OG0006167, OG0006982, OG0003320, OG0003368, OG0001378, OG0007823, OG0002565, OG0003554, OG0000850, OG0004343, OG0008381, OG0016407, OG0005955, OG0004459, OG0003948, OG0005195, OG0009666, OG0000282, OG0002235, OG0007732, OG0005603, OG0008301, OG0006890, OG0003177, OG0009157, OG0008222, OG0003548, OG0007649, OG0002312, OG0010914, OG0004245, OG0001606, OG0009174, OG0047591, OG0004362, OG0009584, OG0007347, OG0008644, OG0003147, OG0001798, OG0002084, OG0000298, OG0001756, OG0002321, OG0016513, OG0006119, OG0005119, OG0047607, OG0001510, OG0006679, OG0008698, OG0008320, OG0003863, OG0005836, OG0000662, OG0006919, OG0011702, OG0002285, OG0009813, OG0001627, OG0006250, OG0047614, OG0011692, OG0009451, OG0008364, OG0002737, OG0047745, OG0000586, OG0026193, OG0006614, OG0019017, OG0001361, OG0003778, OG0004824, OG0000135, OG0004294, OG0012241, OG0011367, OG0009112, OG0000300, OG0016416, OG0010518, OG0011596, OG0007074, OG0001023, OG0007612, OG0002994, OG0004746, OG0007219, OG0000872, OG0013589
## 4 OG0004246, OG0021513, OG0002904, OG0033168, OG0014398, OG0006163, OG0008719, OG0019224, OG0014404, OG0001209, OG0000910, OG0001311, OG0000025, OG0006944, OG0000145, OG0000505, OG0001374, OG0000219, OG0003064, OG0008829, OG0011330, OG0015711, OG0011176, OG0004787, OG0001767, OG0008460, OG0002345, OG0001382, OG0001890, OG0000685, OG0000054, OG0033196, OG0001523, OG0005198, OG0000418, OG0002138, OG0006081, OG0002353, OG0014247, OG0009823, OG0000250, OG0002541, OG0001459, OG0047540, OG0000349, OG0009199, OG0002052, OG0015302, OG0005120, OG0002493, OG0001333, OG0000230, OG0011983, OG0002088, OG0002358, OG0010240, OG0008657, OG0005197, OG0003381, OG0010846, OG0001602, OG0000437, OG0006899, OG0009632, OG0001160, OG0000825, OG0002810, OG0002347, OG0004869, OG0005298, OG0003677, OG0009309, OG0002019, OG0003790, OG0009183, OG0001246, OG0000174, OG0000045, OG0003013, OG0048110, OG0048111, OG0047319, OG0000879, OG0007188, OG0003847, OG0010448, OG0000352, OG0006974, OG0002646, OG0008414, OG0000082, OG0001127, OG0006264, OG0000534, OG0001181, OG0001082, OG0000575, OG0006609, OG0003772, OG0026859, OG0033389, OG0006615, OG0006232, OG0008054, OG0007774, OG0000116, OG0011706, OG0048097, OG0008815, OG0007339, OG0000119, OG0007647, OG0033387, OG0004377, OG0009082, OG0003543, OG0009102, OG0004060, OG0004521, OG0001608, OG0004226, OG0007300, OG0017602, OG0011272, OG0000077, OG0015159, OG0005372, OG0003676, OG0004786, OG0003699, OG0004144, OG0006545, OG0001316, OG0010641, OG0000048, OG0001419, OG0003900, OG0001559, OG0006125, OG0004041, OG0001279, OG0008602, OG0003998, OG0001741, OG0005614, OG0047286, OG0001188, OG0008569, OG0005822, OG0009620, OG0000239, OG0005465, OG0006172, OG0047260, OG0005633, OG0004692, OG0007653, OG0005613, OG0002487, OG0010542, OG0008027, OG0001903, OG0007461, OG0002639, OG0009150, OG0002368, OG0003360, OG0014112, OG0004751, OG0006353, OG0002028, OG0017239, OG0008422, OG0000257, OG0009058, OG0000363, OG0006385, OG0026085, OG0009475, OG0003471, OG0002875, OG0000014, OG0004884, OG0003435, OG0006849, OG0005055, OG0006149, OG0010827, OG0000724, OG0005079, OG0000128, OG0005270, OG0000391, OG0004907, OG0009724, OG0005699, OG0000696, OG0008567, OG0004202, OG0004184, OG0008673, OG0007817, OG0002761, OG0000102, OG0007314, OG0007541, OG0002376, OG0005124, OG0001018, OG0002722, OG0000548, OG0003135, OG0006225, OG0006001, OG0004644, OG0020520, OG0003004, OG0002975, OG0001673, OG0000124, OG0004407, OG0002103, OG0007177, OG0006747, OG0007277, OG0008649, OG0009723, OG0013721, OG0000312, OG0047278, OG0007309, OG0018375, OG0006373, OG0005175, OG0008311, OG0010226, OG0011256, OG0007335, OG0007450, OG0003292, OG0001490, OG0012810, OG0006136, OG0001177, OG0000429, OG0004645, OG0012872, OG0003625, OG0003859, OG0008028, OG0008767, OG0013481, OG0000121, OG0005439, OG0033134, OG0010155, OG0008658, OG0000288, OG0004082, OG0047398, OG0007304, OG0003340, OG0005283, OG0004209, OG0002414, OG0047402, OG0004014, OG0000994, OG0004228, OG0001103, OG0006259, OG0018867, OG0001463, OG0009125, OG0021810, OG0003365, OG0009396, OG0000279, OG0008580, OG0000185, OG0017301, OG0018987, OG0047412, OG0000037, OG0002267, OG0003855, OG0008809, OG0000676, OG0004757, OG0002835, OG0001278, OG0003370, OG0010568, OG0000325, OG0007080, OG0000175, OG0001199, OG0000527, OG0004572, OG0003679, OG0003309, OG0001344, OG0008651, OG0047742, OG0001439, OG0000214, OG0033031, OG0011442, OG0000020, OG0010154, OG0019411, OG0001722, OG0010333, OG0006702, OG0020483, OG0003670, OG0005445, OG0008682, OG0019322, OG0009216, OG0002410, OG0002740, OG0007691, OG0003536, OG0005512, OG0026163, OG0007183, OG0047882, OG0033329, OG0000341, OG0018008, OG0015529, OG0013558, OG0008510, OG0003885, OG0005950, OG0007317, OG0001857, OG0004731, OG0001864, OG0000317, OG0006379, OG0005677, OG0000434, OG0005045, OG0004942, OG0012249, OG0000247, OG0001016, OG0005855, OG0001159, OG0000293, OG0001211, OG0010890, OG0047706, OG0001753, OG0033238, OG0000031, OG0010107, OG0047708, OG0047709, OG0008009, OG0004375, OG0018953, OG0014657, OG0000567, OG0003158, OG0047115, OG0000016, OG0006138, OG0007042, OG0005590, OG0007827, OG0007496, OG0001661, OG0003284, OG0000523, OG0008305, OG0026053, OG0004616, OG0000390, OG0011708, OG0004622, OG0001656, OG0001328, OG0002912, OG0001400, OG0047159, OG0001633, OG0007495, OG0018083, OG0001551, OG0003005, OG0003702, OG0005150, OG0007667, OG0009675, OG0002531, OG0007981, OG0005173, OG0002555, OG0006338, OG0009164, OG0006572, OG0005837, OG0006199, OG0047280, OG0002435, OG0007968, OG0000477, OG0010610, OG0000780, OG0007392, OG0000113, OG0006003, OG0001252, OG0001730, OG0033167, OG0003507, OG0008760, OG0002193, OG0001235, OG0005900, OG0001625, OG0005506, OG0019476, OG0033376, OG0026262, OG0019029, OG0000810, OG0009335, OG0004160, OG0007119, OG0000109, OG0005144, OG0009421, OG0001670, OG0000925, OG0008519, OG0005294, OG0001986, OG0048045, OG0026170, OG0000392, OG0002164, OG0015720, OG0000732, OG0006106, OG0009092, OG0033373, OG0006316, OG0016075, OG0007793, OG0011977, OG0004860, OG0048067, OG0008872, OG0000066, OG0002754, OG0005191, OG0000111, OG0010545, OG0006341, OG0048068, OG0007795, OG0005277, OG0006876, OG0007847, OG0007509, OG0004389, OG0009293, OG0002789, OG0013604, OG0000588, OG0002502, OG0001337, OG0000067, OG0002562, OG0000130, OG0021764, OG0047671, OG0002224, OG0000972, OG0001676, OG0017364, OG0009816, OG0002212, OG0008876, OG0001858, OG0012114, OG0006990, OG0001087, OG0000400, OG0015679, OG0047676, OG0047677, OG0047678, OG0015693, OG0001360, OG0010193, OG0000727, OG0002359, OG0004296, OG0002196, OG0001493, OG0007075, OG0003388, OG0003813, OG0033230, OG0002655, OG0000316, OG0001936, OG0000218, OG0003383, OG0009424, OG0007220, OG0003492, OG0001820, OG0006276, OG0003355, OG0000063, OG0000034, OG0009824, OG0021840, OG0010181, OG0000188, OG0009152, OG0002153, OG0003176, OG0008857, OG0004921, OG0007661, OG0005766, OG0009135, OG0005142, OG0005895, OG0000326, OG0006745, OG0003716, OG0000313, OG0007338, OG0002948, OG0005242, OG0001404, OG0006683, OG0003925, OG0015535, OG0013496, OG0005418, OG0011721, OG0002779, OG0008547, OG0008079, OG0000953, OG0026255, OG0007122, OG0004121, OG0002522, OG0013239, OG0025898, OG0018068, OG0002058, OG0007806, OG0000572, OG0011766, OG0001960, OG0003926, OG0011111, OG0002418, OG0006983, OG0004808, OG0001869, OG0011654, OG0000894, OG0000681, OG0001410, OG0005750, OG0000997, OG0002712, OG0018973, OG0000337, OG0002537, OG0012840, OG0006537, OG0019357, OG0018162, OG0001233, OG0001287, OG0004402, OG0008328, OG0001704, OG0007006, OG0007044, OG0021812, OG0008075, OG0010104, OG0021772, OG0011698, OG0011672, OG0000310, OG0016436, OG0000007, OG0001871, OG0002401, OG0008977, OG0000322, OG0007452, OG0047857, OG0005570, OG0006668, OG0000019, OG0047860, OG0031954, OG0004707, OG0006365, OG0004587, OG0006881, OG0007298, OG0008432, OG0002622, OG0002095, OG0009165, OG0005423, OG0003157, OG0007800, OG0026226, OG0001519, OG0003294, OG0013488, OG0033314, OG0007010, OG0004697, OG0011168, OG0003953, OG0004781, OG0007628, OG0008043, OG0047849, OG0019213, OG0003011, OG0017282, OG0001186, OG0005609, OG0007929, OG0006262, OG0005135, OG0047448, OG0004482, OG0005245, OG0001396, OG0012225, OG0001095, OG0000895, OG0001956, OG0047453, OG0000406, OG0010920, OG0005235, OG0003884, OG0004471, OG0005272, OG0002452, OG0016427, OG0003481, OG0010861, OG0000848, OG0005979, OG0000006, OG0003134, OG0004758, OG0000142, OG0002517, OG0002149, OG0005413, OG0015531, OG0005412, OG0007633, OG0005383, OG0004628, OG0006165, OG0007527, OG0002787, OG0002491, OG0001666, OG0004403, OG0001678, OG0002336, OG0000619, OG0001790, OG0004627, OG0008280, OG0011759, OG0001584, OG0047486, OG0000011, OG0007089, OG0001555, OG0001747, OG0047488, OG0002725, OG0002459, OG0008866, OG0005373, OG0005007, OG0000084, OG0000948, OG0007773, OG0008257, OG0001139, OG0002998, OG0006993, OG0004803, OG0002403, OG0001511, OG0001719, OG0000731, OG0000364, OG0008323, OG0005795, OG0007497, OG0007400, OG0000256, OG0003054, OG0047520, OG0047499, OG0001401, OG0007070, OG0023006, OG0005714, OG0001001, OG0006094, OG0009043, OG0006256, OG0026187, OG0000213, OG0001496, OG0033243, OG0047087, OG0005103, OG0006389, OG0000137, OG0010223, OG0014825, OG0002293, OG0000209, OG0002841, OG0005040, OG0012719, OG0000090, OG0005448, OG0000716, OG0012716, OG0002063, OG0013613, OG0026025, OG0006387, OG0007672, OG0000040, OG0001668, OG0014127, OG0003189, OG0002629, OG0006960, OG0002879, OG0000638, OG0004820, OG0007399, OG0002499, OG0001780, OG0000074, OG0033032, OG0002135, OG0001170, OG0008595, OG0008275, OG0001554, OG0002685, OG0006694, OG0011208, OG0007284, OG0003549, OG0047110, OG0007848, OG0000890, OG0000909, OG0009839, OG0047086, OG0011032, OG0033127, OG0002022, OG0001306, OG0006686, OG0019509, OG0001412, OG0003376, OG0000254, OG0001613, OG0048026, OG0002656, OG0012829, OG0016442, OG0004935, OG0001743, OG0004710, OG0002070, OG0008317, OG0003557, OG0001314, OG0004345, OG0001802, OG0000453, OG0000064, OG0007660, OG0009093, OG0001305, OG0007155, OG0008318, OG0017006, OG0000529, OG0003210, OG0018984, OG0003868, OG0003036, OG0013770, OG0000412, OG0000236, OG0007329, OG0000151, OG0009658, OG0003737, OG0002318, OG0009568, OG0005974, OG0009129, OG0000574, OG0006376, OG0006570, OG0005522, OG0008947, OG0009168, OG0005919, OG0002628, OG0002751, OG0002217, OG0000592, OG0006987, OG0009182, OG0004909, OG0007239, OG0002855, OG0008276, OG0001571, OG0005201, OG0007417, OG0013029, OG0006908, OG0002508, OG0000201, OG0007820, OG0007316, OG0003872, OG0005857, OG0007487, OG0000323, OG0004514, OG0001582, OG0001220, OG0007736, OG0001914, OG0009544, OG0006308, OG0008524, OG0005488, OG0002799, OG0001451, OG0005377, OG0047192, OG0008284, OG0005816, OG0003128, OG0003387, OG0005815, OG0009565, OG0008411, OG0006634, OG0001525, OG0002479, OG0005915, OG0008870, OG0004776, OG0000721, OG0001799, OG0047174, OG0005321, OG0009411, OG0008074, OG0002297, OG0010956, OG0009571, OG0003639, OG0003291, OG0000344, OG0002344, OG0009582, OG0002211, OG0000943, OG0000643, OG0006604, OG0009180, OG0008837, OG0001164, OG0002728, OG0004988, OG0005535, OG0000800, OG0000168, OG0047166, OG0007775, OG0004789, OG0000847, OG0008797, OG0003927, OG0004788, OG0002166, OG0004995, OG0009195, OG0004914, OG0006096, OG0006010, OG0001720, OG0033075, OG0012827, OG0000241, OG0001216, OG0001234, OG0008046, OG0004903, OG0000402, OG0003371, OG0011266, OG0007243, OG0002354, OG0003251, OG0004216, OG0002908, OG0007324, OG0006513, OG0003769, OG0019446, OG0004460, OG0002034, OG0007253, OG0006180, OG0002590, OG0011764, OG0004312, OG0007111, OG0012858, OG0047245, OG0000612, OG0034480, OG0001487, OG0009140, OG0000115, OG0011207, OG0001405, OG0008496, OG0000546, OG0002461, OG0012180, OG0002421, OG0002716, OG0001868, OG0000235, OG0006098, OG0008861, OG0007160, OG0007745, OG0003480, OG0016997, OG0002668, OG0004872, OG0006118, OG0004842, OG0001572, OG0003048, OG0024547, OG0002201, OG0007114, OG0011718, OG0006100, OG0003574, OG0000978, OG0009485, OG0009587, OG0000359, OG0000273, OG0009091, OG0005524, OG0002083, OG0005898, OG0007424, OG0004527, OG0001953, OG0008389, OG0000781, OG0007836, OG0006212, OG0000056, OG0008598, OG0047335, OG0047313, OG0013674, OG0007048, OG0047339, OG0005651, OG0009896, OG0000818, OG0000777, OG0006678, OG0007601, OG0000311, OG0003567, OG0006566, OG0002902, OG0005701, OG0001883, OG0008350, OG0002724, OG0002433, OG0007977, OG0001776, OG0006070, OG0015528, OG0033109, OG0002234, OG0017003, OG0003356, OG0005395, OG0008588, OG0005831, OG0000297, OG0032781, OG0004405, OG0004125, OG0004504, OG0002602, OG0012844, OG0002090, OG0005208, OG0047325, OG0007019, OG0000046, OG0004252, OG0008577, OG0004738, OG0007479, OG0004883, OG0007801, OG0002286, OG0006642, OG0000623, OG0002228, OG0000083, OG0000431, OG0000159, OG0008254, OG0004340, OG0004605, OG0005134, OG0011055, OG0002888, OG0002666, OG0004559, OG0001452, OG0013656, OG0003330, OG0006243, OG0015166, OG0003854, OG0007457, OG0008252, OG0005752, OG0026896, OG0000573, OG0010209, OG0008227, OG0011694, OG0005583, OG0001136, OG0047622, OG0009935, OG0008250, OG0011241, OG0014001, OG0001614, OG0004270, OG0008302, OG0023995, OG0001030, OG0015805, OG0006770, OG0003115, OG0011774, OG0002042, OG0006015, OG0011635, OG0002246, OG0020445, OG0003239, OG0004477, OG0002295, OG0005824, OG0004960, OG0014681, OG0033045, OG0002853, OG0009611, OG0001768, OG0008875, OG0003820, OG0003470, OG0047628, OG0001031, OG0004311, OG0000679, OG0006302, OG0001503, OG0008016, OG0001728, OG0026199, OG0026211, OG0007176, OG0009633, OG0007058, OG0010251, OG0047818, OG0000183, OG0008581, OG0003390, OG0007097, OG0003266, OG0000242, OG0001194, OG0047794, OG0009580, OG0004944, OG0002490, OG0005429, OG0007808, OG0000858, OG0015744, OG0008394, OG0001495, OG0007928, OG0001632, OG0005475, OG0006342, OG0002750, OG0001079, OG0007469, OG0006297, OG0002756, OG0006322, OG0005937, OG0004349, OG0003666, OG0008094, OG0009872, OG0011294, OG0000774, OG0000360, OG0006550, OG0001270, OG0004547, OG0005600, OG0007516, OG0009314, OG0014584, OG0014695, OG0000536, OG0017189, OG0004244, OG0008864, OG0002715, OG0006933, OG0005086, OG0003265, OG0003441, OG0008011, OG0000861, OG0001247, OG0003447, OG0047966, OG0001852, OG0003514, OG0007909, OG0033353, OG0000664, OG0010130, OG0003842, OG0021853, OG0004621, OG0005659, OG0002155, OG0002973, OG0000097, OG0021836, OG0003358, OG0006997, OG0002989, OG0005786, OG0001356, OG0006676, OG0005568, OG0001369, OG0005599, OG0000415, OG0002986, OG0005772, OG0007610, OG0000790, OG0001663, OG0018205, OG0001317, OG0002209, OG0014490, OG0000456, OG0001643, OG0047723, OG0006053, OG0002545, OG0024464, OG0005625, OG0007170, OG0003611, OG0009430, OG0010930, OG0000601, OG0006363, OG0007778, OG0006167, OG0006982, OG0003320, OG0003368, OG0001378, OG0007823, OG0002565, OG0003554, OG0000850, OG0004343, OG0008381, OG0016407, OG0005955, OG0004459, OG0003948, OG0005195, OG0009666, OG0000282, OG0002235, OG0007732, OG0005603, OG0008301, OG0006890, OG0003177, OG0009157, OG0008222, OG0003548, OG0007649, OG0002312, OG0010914, OG0004245, OG0001606, OG0009174, OG0047591, OG0004362, OG0009584, OG0007347, OG0008644, OG0003147, OG0001798, OG0002084, OG0000298, OG0001756, OG0002321, OG0016513, OG0006119, OG0005119, OG0047607, OG0001510, OG0006679, OG0008698, OG0008320, OG0003863, OG0005836, OG0000662, OG0006919, OG0011702, OG0002285, OG0009813, OG0001627, OG0006250, OG0047614, OG0011692, OG0009451, OG0008364, OG0002737, OG0047745, OG0000586, OG0026193, OG0006614, OG0019017, OG0001361, OG0003778, OG0004824, OG0000135, OG0004294, OG0012241, OG0011367, OG0009112, OG0000300, OG0016416, OG0010518, OG0011596, OG0007074, OG0001023, OG0007612, OG0002994, OG0004746, OG0007219, OG0000872, OG0013589
## 5 OG0004246, OG0021513, OG0002904, OG0033168, OG0014398, OG0006163, OG0008719, OG0019224, OG0014404, OG0001209, OG0000910, OG0001311, OG0000025, OG0006944, OG0000145, OG0000505, OG0001374, OG0003064, OG0008829, OG0011330, OG0015711, OG0011176, OG0004787, OG0001767, OG0008460, OG0002345, OG0001382, OG0001890, OG0000054, OG0033196, OG0001523, OG0005198, OG0000418, OG0002138, OG0006081, OG0002353, OG0014247, OG0009823, OG0000250, OG0002541, OG0001459, OG0047540, OG0000349, OG0009199, OG0002052, OG0015302, OG0005120, OG0002493, OG0001333, OG0000230, OG0011983, OG0002088, OG0002358, OG0010240, OG0008657, OG0005197, OG0003381, OG0010846, OG0001602, OG0000437, OG0006899, OG0009632, OG0001160, OG0000825, OG0002810, OG0002347, OG0004869, OG0005298, OG0003677, OG0009309, OG0002019, OG0003790, OG0009183, OG0001246, OG0000174, OG0000045, OG0003013, OG0048110, OG0048111, OG0047319, OG0007188, OG0003847, OG0010448, OG0006974, OG0002646, OG0008414, OG0000082, OG0001127, OG0006264, OG0000534, OG0001181, OG0001082, OG0000575, OG0006609, OG0003772, OG0026859, OG0033389, OG0006615, OG0006232, OG0008054, OG0007774, OG0000116, OG0011706, OG0048097, OG0008815, OG0007339, OG0000119, OG0007647, OG0033387, OG0004377, OG0009082, OG0003543, OG0009102, OG0004060, OG0004521, OG0001608, OG0004226, OG0007300, OG0017602, OG0011272, OG0000077, OG0015159, OG0005372, OG0003676, OG0004786, OG0003699, OG0004144, OG0006545, OG0001316, OG0010641, OG0000048, OG0001419, OG0003900, OG0001559, OG0006125, OG0004041, OG0001279, OG0008602, OG0003998, OG0001741, OG0005614, OG0047286, OG0001188, OG0008569, OG0005822, OG0009620, OG0000239, OG0005465, OG0006172, OG0047260, OG0005633, OG0004692, OG0007653, OG0005613, OG0002487, OG0010542, OG0008027, OG0001903, OG0007461, OG0002639, OG0009150, OG0002368, OG0003360, OG0014112, OG0004751, OG0006353, OG0002028, OG0017239, OG0008422, OG0000257, OG0009058, OG0000363, OG0006385, OG0026085, OG0009475, OG0003471, OG0002875, OG0000014, OG0004884, OG0003435, OG0006849, OG0005055, OG0006149, OG0010827, OG0000724, OG0005079, OG0000128, OG0005270, OG0000391, OG0004907, OG0009724, OG0005699, OG0000696, OG0008567, OG0004202, OG0004184, OG0008673, OG0007817, OG0002761, OG0000102, OG0007314, OG0007541, OG0002376, OG0005124, OG0001018, OG0002722, OG0000548, OG0003135, OG0006225, OG0006001, OG0004644, OG0020520, OG0003004, OG0002975, OG0001673, OG0000124, OG0004407, OG0002103, OG0007177, OG0006747, OG0007277, OG0008649, OG0009723, OG0013721, OG0000312, OG0047278, OG0007309, OG0018375, OG0006373, OG0005175, OG0008311, OG0010226, OG0011256, OG0007335, OG0007450, OG0003292, OG0001490, OG0012810, OG0006136, OG0001177, OG0000429, OG0004645, OG0012872, OG0003625, OG0003859, OG0008028, OG0008767, OG0013481, OG0000121, OG0005439, OG0033134, OG0010155, OG0008658, OG0000288, OG0004082, OG0047398, OG0007304, OG0003340, OG0005283, OG0004209, OG0002414, OG0047402, OG0004014, OG0000994, OG0004228, OG0001103, OG0006259, OG0018867, OG0001463, OG0009125, OG0021810, OG0003365, OG0009396, OG0000279, OG0008580, OG0000185, OG0017301, OG0018987, OG0047412, OG0000037, OG0002267, OG0003855, OG0008809, OG0000676, OG0004757, OG0002835, OG0001278, OG0003370, OG0010568, OG0000325, OG0007080, OG0000175, OG0001199, OG0000527, OG0004572, OG0003679, OG0003309, OG0001344, OG0008651, OG0047742, OG0001439, OG0000214, OG0033031, OG0011442, OG0000020, OG0010154, OG0019411, OG0001722, OG0010333, OG0006702, OG0020483, OG0003670, OG0005445, OG0008682, OG0019322, OG0009216, OG0002410, OG0002740, OG0007691, OG0003536, OG0005512, OG0026163, OG0007183, OG0047882, OG0033329, OG0000341, OG0018008, OG0015529, OG0013558, OG0008510, OG0003885, OG0005950, OG0007317, OG0001857, OG0004731, OG0001864, OG0000317, OG0006379, OG0005677, OG0000434, OG0005045, OG0004942, OG0012249, OG0000247, OG0001016, OG0005855, OG0001159, OG0000293, OG0001211, OG0010890, OG0047706, OG0001753, OG0033238, OG0000031, OG0010107, OG0047708, OG0047709, OG0008009, OG0004375, OG0018953, OG0014657, OG0000567, OG0003158, OG0047115, OG0006138, OG0007042, OG0005590, OG0007827, OG0007496, OG0001661, OG0003284, OG0000523, OG0008305, OG0026053, OG0004616, OG0000390, OG0011708, OG0004622, OG0001656, OG0001328, OG0002912, OG0001400, OG0047159, OG0001633, OG0007495, OG0018083, OG0001551, OG0003005, OG0003702, OG0005150, OG0007667, OG0009675, OG0002531, OG0007981, OG0005173, OG0002555, OG0006338, OG0009164, OG0006572, OG0005837, OG0006199, OG0047280, OG0002435, OG0007968, OG0000477, OG0010610, OG0000780, OG0007392, OG0000113, OG0006003, OG0001252, OG0001730, OG0033167, OG0003507, OG0008760, OG0002193, OG0001235, OG0005900, OG0001625, OG0005506, OG0019476, OG0033376, OG0026262, OG0019029, OG0000810, OG0009335, OG0004160, OG0007119, OG0000109, OG0005144, OG0009421, OG0001670, OG0000925, OG0008519, OG0005294, OG0001986, OG0048045, OG0026170, OG0000392, OG0002164, OG0015720, OG0000732, OG0006106, OG0009092, OG0033373, OG0006316, OG0016075, OG0007793, OG0011977, OG0004860, OG0048067, OG0008872, OG0000066, OG0002754, OG0005191, OG0000111, OG0010545, OG0006341, OG0048068, OG0007795, OG0005277, OG0006876, OG0007847, OG0007509, OG0004389, OG0009293, OG0002789, OG0013604, OG0000588, OG0002502, OG0001337, OG0000067, OG0002562, OG0000130, OG0021764, OG0000152, OG0047671, OG0002224, OG0000972, OG0001676, OG0017364, OG0009816, OG0002212, OG0008876, OG0001858, OG0012114, OG0006990, OG0001087, OG0000400, OG0015679, OG0047676, OG0047677, OG0047678, OG0015693, OG0001360, OG0010193, OG0000727, OG0002359, OG0004296, OG0002196, OG0001493, OG0007075, OG0003388, OG0003813, OG0033230, OG0002655, OG0000316, OG0001936, OG0000218, OG0003383, OG0009424, OG0007220, OG0003492, OG0001820, OG0006276, OG0003355, OG0000063, OG0000034, OG0009824, OG0021840, OG0010181, OG0000188, OG0009152, OG0002153, OG0003176, OG0008857, OG0004921, OG0007661, OG0005766, OG0009135, OG0005142, OG0005895, OG0000326, OG0006745, OG0003716, OG0000313, OG0007338, OG0002948, OG0005242, OG0001404, OG0006683, OG0003925, OG0015535, OG0013496, OG0005418, OG0011721, OG0002779, OG0008547, OG0008079, OG0000953, OG0026255, OG0007122, OG0004121, OG0002522, OG0013239, OG0025898, OG0018068, OG0002058, OG0007806, OG0000572, OG0011766, OG0001960, OG0003926, OG0011111, OG0002418, OG0006983, OG0004808, OG0001869, OG0011654, OG0000894, OG0000681, OG0001410, OG0005750, OG0000997, OG0002712, OG0018973, OG0000337, OG0002537, OG0012840, OG0006537, OG0019357, OG0018162, OG0001233, OG0001287, OG0004402, OG0008328, OG0001704, OG0007006, OG0007044, OG0021812, OG0008075, OG0010104, OG0021772, OG0011698, OG0011672, OG0000310, OG0016436, OG0000007, OG0001871, OG0002401, OG0008977, OG0000322, OG0007452, OG0047857, OG0005570, OG0006668, OG0047860, OG0031954, OG0004707, OG0006365, OG0004587, OG0006881, OG0007298, OG0008432, OG0002622, OG0002095, OG0009165, OG0005423, OG0003157, OG0007800, OG0026226, OG0001519, OG0003294, OG0013488, OG0033314, OG0007010, OG0004697, OG0011168, OG0003953, OG0004781, OG0007628, OG0008043, OG0047849, OG0019213, OG0003011, OG0017282, OG0001186, OG0005609, OG0007929, OG0006262, OG0005135, OG0047448, OG0004482, OG0005245, OG0001396, OG0012225, OG0001095, OG0000895, OG0001956, OG0047453, OG0000406, OG0010920, OG0005235, OG0003884, OG0004471, OG0005272, OG0002452, OG0016427, OG0003481, OG0010861, OG0000848, OG0005979, OG0003134, OG0004758, OG0000142, OG0002517, OG0002149, OG0005413, OG0015531, OG0005412, OG0007633, OG0005383, OG0004628, OG0006165, OG0007527, OG0002787, OG0002491, OG0001666, OG0004403, OG0001678, OG0002336, OG0000619, OG0001790, OG0004627, OG0008280, OG0011759, OG0001584, OG0047486, OG0000011, OG0007089, OG0001555, OG0001747, OG0047488, OG0002725, OG0002459, OG0008866, OG0005373, OG0005007, OG0000084, OG0000948, OG0007773, OG0008257, OG0001139, OG0002998, OG0006993, OG0003047, OG0004803, OG0002403, OG0001511, OG0000731, OG0000364, OG0008323, OG0005795, OG0007497, OG0007400, OG0000256, OG0003054, OG0047520, OG0047499, OG0001401, OG0007070, OG0023006, OG0005714, OG0001001, OG0006094, OG0009043, OG0006256, OG0026187, OG0000213, OG0001496, OG0033243, OG0047087, OG0005103, OG0006389, OG0000137, OG0010223, OG0014825, OG0002293, OG0000209, OG0002841, OG0005040, OG0012719, OG0000090, OG0005448, OG0000716, OG0012716, OG0002063, OG0013613, OG0026025, OG0006387, OG0007672, OG0000040, OG0001668, OG0014127, OG0003189, OG0002629, OG0006960, OG0002879, OG0000638, OG0004820, OG0007399, OG0002499, OG0001780, OG0000074, OG0033032, OG0002135, OG0001170, OG0008595, OG0008275, OG0001554, OG0002685, OG0006694, OG0011208, OG0007284, OG0003549, OG0047110, OG0007848, OG0000890, OG0000909, OG0009839, OG0047086, OG0011032, OG0033127, OG0002022, OG0001306, OG0006686, OG0019509, OG0001412, OG0003376, OG0000254, OG0001613, OG0048026, OG0002656, OG0012829, OG0016442, OG0004935, OG0001743, OG0004710, OG0002070, OG0008317, OG0003557, OG0001314, OG0004345, OG0001802, OG0000453, OG0000064, OG0007660, OG0009093, OG0001305, OG0007155, OG0008318, OG0017006, OG0000529, OG0003210, OG0018984, OG0003868, OG0003036, OG0013770, OG0000412, OG0000236, OG0007329, OG0000151, OG0009658, OG0003737, OG0002318, OG0009568, OG0005974, OG0009129, OG0000574, OG0006376, OG0006570, OG0005522, OG0008947, OG0009168, OG0005919, OG0002628, OG0002751, OG0002217, OG0000592, OG0006987, OG0009182, OG0004909, OG0007239, OG0002855, OG0008276, OG0001571, OG0005201, OG0007417, OG0013029, OG0006908, OG0002508, OG0000201, OG0007820, OG0007316, OG0003872, OG0005857, OG0007487, OG0000323, OG0004514, OG0001582, OG0001220, OG0007736, OG0001914, OG0009544, OG0006308, OG0008524, OG0005488, OG0002799, OG0001451, OG0005377, OG0047192, OG0008284, OG0005816, OG0003128, OG0003387, OG0005815, OG0007478, OG0009565, OG0008411, OG0006634, OG0001525, OG0002479, OG0005915, OG0008870, OG0004776, OG0000721, OG0001799, OG0047174, OG0005321, OG0009411, OG0008074, OG0002297, OG0010956, OG0009571, OG0003639, OG0003291, OG0000344, OG0002344, OG0009582, OG0002211, OG0000943, OG0000643, OG0006604, OG0009180, OG0008837, OG0001164, OG0002728, OG0004988, OG0005535, OG0000800, OG0000168, OG0047166, OG0007775, OG0004789, OG0000847, OG0008797, OG0003927, OG0004788, OG0002166, OG0004995, OG0009195, OG0004914, OG0006096, OG0006010, OG0001720, OG0033075, OG0012827, OG0000241, OG0001216, OG0001234, OG0008046, OG0004903, OG0000402, OG0003371, OG0011266, OG0007243, OG0002354, OG0003251, OG0004216, OG0002908, OG0007324, OG0006513, OG0003769, OG0019446, OG0004460, OG0002034, OG0007253, OG0006180, OG0002590, OG0011764, OG0004312, OG0007111, OG0012858, OG0047245, OG0000612, OG0034480, OG0001487, OG0009140, OG0000115, OG0011207, OG0001405, OG0008496, OG0000546, OG0002461, OG0012180, OG0002421, OG0002716, OG0001868, OG0000235, OG0006098, OG0008861, OG0007160, OG0007745, OG0003480, OG0016997, OG0002668, OG0004872, OG0006118, OG0004842, OG0001572, OG0003048, OG0024547, OG0002201, OG0007114, OG0011718, OG0006100, OG0003574, OG0000978, OG0009485, OG0009587, OG0000359, OG0000273, OG0009091, OG0005524, OG0002083, OG0005898, OG0007424, OG0004527, OG0001953, OG0008389, OG0000781, OG0007836, OG0006212, OG0008598, OG0047335, OG0047313, OG0013674, OG0007048, OG0047339, OG0005651, OG0009896, OG0000818, OG0006678, OG0007601, OG0000311, OG0003567, OG0006566, OG0002902, OG0005701, OG0001883, OG0008350, OG0002724, OG0002433, OG0007977, OG0001776, OG0006070, OG0015528, OG0033109, OG0002234, OG0017003, OG0003356, OG0005395, OG0008588, OG0005831, OG0032781, OG0004405, OG0004125, OG0004504, OG0002602, OG0012844, OG0002090, OG0005208, OG0047325, OG0007019, OG0000046, OG0004252, OG0008577, OG0004738, OG0007479, OG0004883, OG0007801, OG0002286, OG0006642, OG0000623, OG0002228, OG0000083, OG0000431, OG0000159, OG0008254, OG0004340, OG0004605, OG0005134, OG0011055, OG0002888, OG0002666, OG0004559, OG0001452, OG0013656, OG0003330, OG0006243, OG0015166, OG0003854, OG0007457, OG0008252, OG0005752, OG0026896, OG0000573, OG0010209, OG0008227, OG0011694, OG0005583, OG0001136, OG0047622, OG0009935, OG0008250, OG0011241, OG0014001, OG0001614, OG0004270, OG0008302, OG0023995, OG0001030, OG0015805, OG0006770, OG0003115, OG0011774, OG0002042, OG0006015, OG0011635, OG0002246, OG0020445, OG0003239, OG0004477, OG0002295, OG0005824, OG0004960, OG0014681, OG0033045, OG0002853, OG0009611, OG0001768, OG0008875, OG0003820, OG0003470, OG0047628, OG0001031, OG0004311, OG0000679, OG0006302, OG0001503, OG0008016, OG0001728, OG0026199, OG0026211, OG0007176, OG0009633, OG0007058, OG0010251, OG0047818, OG0000183, OG0008581, OG0003390, OG0007097, OG0003266, OG0000242, OG0001194, OG0047794, OG0009580, OG0004944, OG0002490, OG0005429, OG0007808, OG0000858, OG0015744, OG0008394, OG0001495, OG0007928, OG0001632, OG0005475, OG0006342, OG0002750, OG0001079, OG0007469, OG0006297, OG0002756, OG0006322, OG0005937, OG0004349, OG0003666, OG0008094, OG0009872, OG0011294, OG0000774, OG0000360, OG0006550, OG0001270, OG0004547, OG0005600, OG0007516, OG0009314, OG0014584, OG0014695, OG0000536, OG0017189, OG0004244, OG0008864, OG0002715, OG0006933, OG0005086, OG0003265, OG0003441, OG0008011, OG0000861, OG0001247, OG0003447, OG0047966, OG0001852, OG0003514, OG0007909, OG0033353, OG0000664, OG0010130, OG0003842, OG0021853, OG0004621, OG0005659, OG0002155, OG0002973, OG0000097, OG0021836, OG0003358, OG0006997, OG0002989, OG0005786, OG0001356, OG0006676, OG0005568, OG0001369, OG0005599, OG0000415, OG0002986, OG0005772, OG0007610, OG0000790, OG0001663, OG0018205, OG0001232, OG0001317, OG0002209, OG0014490, OG0000456, OG0001643, OG0047723, OG0006053, OG0002545, OG0024464, OG0005625, OG0007170, OG0003611, OG0009430, OG0010930, OG0000601, OG0006363, OG0007778, OG0006167, OG0006982, OG0003320, OG0003368, OG0001378, OG0007823, OG0002565, OG0003554, OG0000850, OG0004343, OG0008381, OG0016407, OG0005955, OG0004459, OG0003948, OG0005195, OG0009666, OG0000282, OG0002235, OG0007732, OG0005603, OG0008301, OG0006890, OG0003177, OG0009157, OG0008222, OG0003548, OG0007649, OG0002312, OG0010914, OG0004245, OG0001606, OG0009174, OG0047591, OG0004362, OG0009584, OG0007347, OG0008644, OG0003147, OG0001798, OG0002084, OG0000298, OG0001756, OG0002321, OG0016513, OG0006119, OG0005119, OG0047607, OG0001510, OG0006679, OG0008698, OG0008320, OG0003863, OG0005836, OG0000662, OG0006919, OG0011702, OG0002285, OG0009813, OG0001627, OG0006250, OG0047614, OG0011692, OG0009451, OG0008364, OG0002737, OG0047745, OG0000586, OG0026193, OG0006614, OG0019017, OG0001361, OG0003778, OG0004824, OG0000135, OG0004294, OG0012241, OG0011367, OG0009112, OG0000300, OG0016416, OG0010518, OG0011596, OG0007074, OG0001023, OG0007612, OG0002994, OG0004746, OG0007219, OG0000872, OG0013589
## 6 OG0004246, OG0021513, OG0002904, OG0033168, OG0014398, OG0006163, OG0008719, OG0019224, OG0014404, OG0001209, OG0000910, OG0001311, OG0000025, OG0006944, OG0000145, OG0000505, OG0001374, OG0000219, OG0003064, OG0008829, OG0011330, OG0015711, OG0011176, OG0004787, OG0001767, OG0008460, OG0002345, OG0001382, OG0001890, OG0000685, OG0033196, OG0001523, OG0005198, OG0000418, OG0002138, OG0006081, OG0002353, OG0014247, OG0009823, OG0000250, OG0002541, OG0001459, OG0047540, OG0000349, OG0009199, OG0002052, OG0015302, OG0005120, OG0002493, OG0001333, OG0000230, OG0011983, OG0002088, OG0002358, OG0010240, OG0008657, OG0005197, OG0003381, OG0010846, OG0001602, OG0000437, OG0006899, OG0009632, OG0001160, OG0000825, OG0002810, OG0002347, OG0004869, OG0005298, OG0003677, OG0009309, OG0002019, OG0003790, OG0009183, OG0001246, OG0000174, OG0000045, OG0003013, OG0048110, OG0048111, OG0047319, OG0000879, OG0007188, OG0003847, OG0010448, OG0000352, OG0006974, OG0002646, OG0008414, OG0001127, OG0006264, OG0000534, OG0001181, OG0001082, OG0000575, OG0006609, OG0003772, OG0026859, OG0033389, OG0006615, OG0006232, OG0008054, OG0007774, OG0000116, OG0011706, OG0048097, OG0008815, OG0007339, OG0000119, OG0007647, OG0033387, OG0004377, OG0009082, OG0003543, OG0009102, OG0004060, OG0004521, OG0001608, OG0004226, OG0007300, OG0017602, OG0011272, OG0000077, OG0015159, OG0005372, OG0003676, OG0004786, OG0003699, OG0004144, OG0006545, OG0001316, OG0010641, OG0000048, OG0001419, OG0003900, OG0001559, OG0006125, OG0004041, OG0001279, OG0008602, OG0003998, OG0001741, OG0005614, OG0047286, OG0001188, OG0008569, OG0005822, OG0009620, OG0000239, OG0005465, OG0006172, OG0047260, OG0005633, OG0004692, OG0007653, OG0005613, OG0002487, OG0010542, OG0008027, OG0007461, OG0002639, OG0009150, OG0002368, OG0003360, OG0014112, OG0004751, OG0006353, OG0002028, OG0017239, OG0008422, OG0000257, OG0009058, OG0000363, OG0006385, OG0026085, OG0009475, OG0003471, OG0002875, OG0004884, OG0003435, OG0006849, OG0005055, OG0006149, OG0010827, OG0000724, OG0005079, OG0000128, OG0005270, OG0000391, OG0004907, OG0009724, OG0005699, OG0000696, OG0008567, OG0004202, OG0004184, OG0008673, OG0007817, OG0002761, OG0000102, OG0007314, OG0007541, OG0002376, OG0005124, OG0001018, OG0002722, OG0000548, OG0003135, OG0006225, OG0006001, OG0004644, OG0020520, OG0003004, OG0002975, OG0001673, OG0000124, OG0004407, OG0002103, OG0007177, OG0006747, OG0007277, OG0008649, OG0009723, OG0013721, OG0000312, OG0047278, OG0007309, OG0018375, OG0006373, OG0005175, OG0008311, OG0010226, OG0011256, OG0007335, OG0007450, OG0003292, OG0001490, OG0012810, OG0006136, OG0001177, OG0000429, OG0004645, OG0012872, OG0003625, OG0003859, OG0008028, OG0008767, OG0013481, OG0000121, OG0005439, OG0033134, OG0010155, OG0008658, OG0000288, OG0004082, OG0047398, OG0007304, OG0003340, OG0005283, OG0004209, OG0002414, OG0047402, OG0004014, OG0000994, OG0004228, OG0001103, OG0006259, OG0018867, OG0001463, OG0009125, OG0021810, OG0009396, 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OG0000016, OG0006138, OG0007042, OG0005590, OG0007827, OG0007496, OG0001661, OG0003284, OG0000523, OG0008305, OG0026053, OG0004616, OG0000390, OG0011708, OG0004622, OG0001656, OG0001328, OG0002912, OG0001400, OG0047159, OG0001633, OG0007495, OG0018083, OG0001551, OG0003005, OG0003702, OG0005150, OG0007667, OG0009675, OG0002531, OG0007981, OG0005173, OG0002555, OG0006338, OG0009164, OG0006572, OG0005837, OG0006199, OG0047280, OG0002435, OG0007968, OG0000477, OG0010610, OG0000780, OG0007392, OG0000113, OG0006003, OG0001252, OG0001730, OG0033167, OG0003507, OG0008760, OG0002193, OG0001235, OG0005900, OG0001625, OG0005506, OG0019476, OG0033376, OG0026262, OG0019029, OG0000810, OG0009335, OG0004160, OG0007119, OG0000109, OG0005144, OG0009421, OG0001670, OG0000925, OG0008519, OG0005294, OG0001986, OG0048045, OG0026170, OG0000392, OG0002164, OG0015720, OG0000732, OG0006106, OG0009092, OG0033373, OG0006316, OG0016075, OG0007793, OG0011977, OG0004860, OG0048067, OG0008872, OG0000066, OG0002754, OG0005191, OG0000111, OG0010545, OG0006341, OG0048068, OG0007795, OG0005277, OG0006876, OG0007847, OG0007509, OG0004389, OG0009293, OG0002789, OG0013604, OG0002502, OG0001337, OG0002562, OG0000130, OG0021764, OG0047671, OG0002224, OG0000972, OG0001676, OG0017364, OG0009816, OG0002212, OG0008876, OG0001858, OG0012114, OG0006990, OG0001087, OG0000400, OG0015679, OG0047676, OG0047677, OG0047678, OG0015693, OG0001360, OG0010193, OG0000727, OG0002359, OG0004296, OG0002196, OG0001493, OG0007075, OG0003388, OG0003813, OG0033230, OG0002655, OG0000316, OG0001936, OG0003383, OG0009424, OG0007220, OG0003492, OG0001820, OG0006276, OG0003355, OG0000063, OG0000034, OG0009824, OG0021840, OG0010181, OG0000188, OG0009152, OG0002153, OG0003176, OG0008857, OG0004921, OG0007661, OG0005766, OG0009135, OG0005142, OG0005895, OG0000326, OG0006745, OG0003716, OG0000313, OG0007338, OG0002948, OG0005242, OG0001404, OG0006683, OG0003925, OG0015535, OG0013496, OG0005418, OG0011721, OG0002779, OG0008547, OG0008079, OG0000953, OG0026255, OG0007122, OG0004121, OG0002522, OG0013239, OG0025898, OG0018068, OG0002058, OG0007806, OG0000572, OG0011766, OG0001960, OG0003926, OG0011111, OG0002418, OG0006983, OG0004808, OG0001869, OG0011654, OG0000894, OG0000681, OG0001410, OG0000997, OG0002712, OG0018973, OG0000337, OG0002537, OG0012840, OG0006537, OG0019357, OG0018162, OG0001233, OG0001287, OG0004402, OG0008328, OG0001704, OG0007006, OG0007044, OG0021812, OG0008075, OG0010104, OG0021772, OG0011698, OG0011672, OG0000310, OG0016436, OG0001871, OG0002401, OG0008977, OG0000322, OG0007452, OG0047857, OG0005570, OG0006668, OG0047860, OG0031954, OG0004707, OG0006365, OG0004587, OG0006881, OG0007298, OG0008432, OG0002622, OG0002095, OG0009165, OG0005423, OG0003157, OG0007800, OG0026226, OG0001519, OG0003294, OG0013488, OG0033314, OG0007010, OG0004697, OG0011168, OG0003953, OG0004781, OG0007628, OG0008043, OG0047849, OG0019213, OG0003011, OG0017282, OG0001186, OG0005609, OG0007929, OG0006262, OG0005135, OG0047448, OG0004482, OG0005245, OG0001396, OG0012225, OG0001095, OG0000895, OG0001956, OG0047453, OG0000406, OG0010920, OG0005235, OG0003884, OG0004471, OG0005272, OG0002452, OG0016427, OG0003481, OG0010861, OG0000848, OG0005979, OG0000006, OG0003134, OG0004758, OG0000142, OG0002517, OG0002149, OG0005413, OG0015531, OG0005412, OG0007633, OG0005383, OG0004628, OG0006165, OG0007527, OG0002787, OG0002491, OG0001666, OG0004403, OG0001678, OG0002336, OG0000619, OG0001790, OG0004627, OG0008280, OG0011759, OG0001584, OG0047486, OG0007089, OG0001555, OG0001747, OG0047488, OG0002725, OG0002459, OG0008866, OG0005373, OG0005007, OG0000084, OG0000948, OG0007773, OG0008257, OG0001139, OG0002998, OG0006993, OG0003047, OG0004803, OG0002403, OG0001511, OG0001719, OG0000731, OG0000364, OG0008323, OG0005795, OG0007497, OG0007400, OG0000256, OG0003054, OG0047520, OG0047499, OG0001401, OG0007070, OG0023006, OG0005714, OG0001001, OG0006094, OG0009043, OG0006256, OG0026187, OG0000213, OG0001496, OG0033243, OG0047087, OG0005103, OG0006389, OG0000137, OG0010223, OG0014825, OG0002293, OG0000209, OG0002841, OG0005040, OG0012719, OG0000090, OG0005448, OG0000716, OG0012716, OG0002063, OG0013613, OG0026025, OG0006387, OG0007672, OG0000040, OG0001668, OG0014127, OG0003189, OG0002629, OG0006960, OG0002879, OG0000638, OG0004820, OG0007399, OG0002499, OG0001780, OG0000074, OG0033032, OG0002135, OG0001170, OG0008595, OG0008275, OG0001554, OG0002685, OG0006694, OG0011208, OG0007284, OG0003549, OG0047110, OG0007848, OG0000890, OG0000909, OG0009839, OG0047086, OG0011032, OG0033127, OG0002022, OG0001306, OG0006686, OG0019509, OG0001412, OG0003376, OG0000254, OG0001613, OG0048026, OG0002656, OG0012829, OG0016442, OG0004935, OG0001743, OG0004710, OG0002070, OG0008317, OG0003557, OG0001314, OG0004345, OG0001802, OG0000453, OG0000064, OG0007660, OG0009093, OG0001305, OG0007155, OG0008318, OG0017006, OG0000529, OG0003210, OG0018984, OG0003868, OG0003036, OG0013770, OG0000412, OG0000236, OG0007329, OG0000151, OG0009658, OG0003737, OG0002318, OG0009568, OG0005974, OG0009129, OG0000574, OG0006376, OG0006570, OG0005522, OG0008947, OG0009168, OG0005919, OG0002628, OG0002751, OG0002217, OG0000592, OG0006987, OG0009182, OG0004909, OG0007239, OG0002855, OG0008276, OG0001571, OG0005201, OG0007417, OG0013029, OG0006908, OG0002508, OG0000201, OG0007820, OG0007316, OG0003872, OG0005857, OG0007487, OG0000323, OG0004514, OG0001582, OG0001220, OG0007736, OG0001914, OG0009544, OG0006308, OG0008524, OG0005488, OG0002799, OG0001451, OG0005377, OG0047192, OG0008284, OG0005816, OG0003128, OG0003387, OG0005815, OG0007478, OG0009565, OG0008411, OG0006634, OG0001525, OG0002479, OG0005915, OG0008870, OG0004776, OG0000721, OG0001799, OG0047174, OG0005321, OG0009411, OG0008074, OG0002297, OG0010956, OG0009571, OG0003639, OG0003291, OG0000344, OG0002344, OG0009582, OG0002211, OG0000943, OG0000643, OG0006604, OG0009180, OG0008837, OG0001164, OG0002728, OG0004988, OG0005535, OG0000800, OG0000168, OG0047166, OG0007775, OG0004789, OG0000847, OG0008797, OG0003927, OG0004788, OG0002166, OG0004995, OG0009195, OG0004914, OG0006096, OG0006010, OG0001720, OG0033075, OG0012827, OG0000241, OG0001216, OG0001234, OG0008046, OG0004903, OG0000402, OG0003371, OG0011266, OG0007243, OG0002354, OG0003251, OG0004216, OG0002908, OG0007324, OG0006513, OG0003769, OG0019446, OG0004460, OG0002034, OG0007253, OG0006180, OG0002590, OG0011764, OG0004312, OG0007111, OG0012858, OG0047245, OG0000612, OG0034480, OG0001487, OG0009140, OG0000115, OG0011207, OG0001405, OG0008496, OG0000546, OG0002461, OG0012180, OG0002421, OG0002716, OG0001868, OG0000235, OG0006098, OG0008861, OG0007160, OG0007745, OG0003480, OG0016997, OG0002668, OG0004872, OG0006118, OG0004842, OG0001572, OG0003048, OG0024547, OG0002201, OG0007114, OG0011718, OG0006100, OG0003574, OG0000978, OG0009485, OG0009587, OG0000359, OG0000273, OG0009091, OG0005524, OG0005898, OG0007424, OG0004527, OG0001953, OG0008389, OG0000781, OG0007836, OG0006212, OG0008598, OG0047335, OG0047313, OG0013674, OG0007048, OG0047339, OG0005651, OG0009896, OG0000818, OG0000777, OG0006678, OG0007601, OG0000311, OG0003567, OG0006566, OG0002902, OG0005701, OG0001883, OG0008350, OG0002724, OG0002433, OG0007977, OG0001776, OG0006070, OG0015528, OG0033109, OG0002234, OG0017003, OG0003356, OG0005395, OG0008588, OG0005831, OG0000297, OG0032781, OG0004405, OG0004125, OG0004504, OG0002602, OG0012844, OG0002090, OG0005208, OG0047325, OG0007019, OG0000046, OG0004252, OG0008577, OG0004738, OG0007479, OG0004883, OG0007801, OG0002286, OG0006642, OG0000623, OG0002228, OG0000431, OG0000159, OG0008254, OG0004340, OG0004605, OG0005134, OG0011055, OG0002888, OG0002666, OG0004559, OG0001452, OG0013656, OG0003330, OG0006243, OG0015166, OG0003854, OG0007457, OG0008252, OG0005752, OG0026896, OG0000573, OG0010209, OG0008227, OG0011694, OG0005583, OG0001136, OG0047622, OG0009935, OG0008250, OG0011241, OG0014001, OG0001614, OG0004270, OG0008302, OG0023995, OG0001030, OG0015805, OG0006770, OG0003115, OG0011774, OG0002042, OG0006015, OG0011635, OG0002246, OG0020445, OG0003239, OG0004477, OG0002295, OG0005824, OG0004960, OG0014681, OG0033045, OG0002853, OG0009611, OG0001768, OG0008875, OG0003820, OG0003470, OG0047628, OG0001031, OG0004311, OG0000679, OG0006302, OG0001503, OG0008016, OG0001728, OG0026199, OG0026211, OG0007176, OG0009633, OG0007058, OG0010251, OG0047818, OG0000183, OG0008581, OG0003390, OG0007097, OG0003266, OG0000242, OG0001194, OG0047794, OG0009580, OG0004944, OG0002490, OG0005429, OG0007808, OG0000858, OG0015744, OG0008394, OG0001495, OG0007928, OG0001632, OG0005475, OG0006342, OG0002750, OG0001079, OG0007469, OG0006297, OG0002756, OG0006322, OG0005937, OG0004349, OG0003666, OG0008094, OG0009872, OG0011294, OG0000774, OG0000360, OG0006550, OG0001270, OG0004547, OG0005600, OG0007516, OG0009314, OG0014584, OG0014695, OG0000536, OG0017189, OG0004244, OG0008864, OG0002715, OG0006933, OG0005086, OG0003265, OG0003441, OG0008011, OG0000861, OG0001247, OG0003447, OG0047966, OG0001852, OG0003514, OG0007909, OG0033353, OG0000664, OG0010130, OG0003842, OG0021853, OG0004621, OG0005659, OG0002155, OG0002973, OG0000097, OG0021836, OG0003358, OG0006997, OG0002989, OG0005786, OG0001356, OG0006676, OG0005568, OG0001369, OG0005599, OG0000415, OG0002986, OG0005772, OG0007610, OG0000790, OG0001663, OG0018205, OG0001232, OG0001317, OG0002209, OG0014490, OG0001643, OG0047723, OG0006053, OG0002545, OG0024464, OG0005625, OG0007170, OG0003611, OG0009430, OG0010930, OG0000601, OG0006363, OG0007778, OG0006167, OG0006982, OG0003320, OG0003368, OG0001378, OG0007823, OG0002565, OG0003554, OG0000850, OG0004343, OG0008381, OG0016407, OG0005955, OG0004459, OG0003948, OG0005195, OG0009666, OG0000282, OG0002235, OG0007732, OG0005603, OG0008301, OG0006890, OG0003177, OG0009157, OG0008222, OG0003548, OG0007649, OG0002312, OG0010914, OG0004245, OG0001606, OG0009174, OG0047591, OG0004362, OG0009584, OG0007347, OG0008644, OG0003147, OG0001798, OG0002084, OG0000298, OG0001756, OG0002321, OG0016513, OG0006119, OG0005119, OG0047607, OG0001510, OG0006679, OG0008698, OG0008320, OG0003863, OG0005836, OG0000662, OG0006919, OG0011702, OG0002285, OG0009813, OG0001627, OG0006250, OG0047614, OG0011692, OG0009451, OG0008364, OG0002737, OG0047745, OG0000586, OG0026193, OG0006614, OG0019017, OG0001361, OG0003778, OG0004824, OG0000135, OG0004294, OG0012241, OG0011367, OG0009112, OG0000300, OG0016416, OG0010518, OG0011596, OG0007074, OG0001023, OG0007612, OG0002994, OG0004746, OG0007219, OG0000872, OG0013589
## 7 OG0004246, OG0021513, OG0002904, OG0033168, OG0014398, OG0006163, OG0008719, OG0019224, OG0014404, OG0001209, OG0000910, OG0001311, OG0000025, OG0006944, OG0000145, OG0000505, OG0001374, OG0000219, OG0003064, OG0008829, OG0011330, OG0015711, OG0011176, OG0004787, OG0001767, OG0008460, OG0002345, OG0001382, OG0001890, OG0000685, OG0000054, OG0033196, OG0001523, OG0005198, OG0000418, OG0002138, OG0006081, OG0002353, OG0014247, OG0009823, OG0000250, OG0002541, OG0001459, OG0047540, OG0000349, OG0009199, OG0002052, OG0015302, OG0005120, OG0002493, OG0001333, OG0000230, OG0011983, OG0002088, OG0002358, OG0010240, OG0008657, OG0005197, OG0003381, OG0010846, OG0001602, OG0000437, OG0006899, OG0009632, OG0001160, OG0000825, OG0002810, OG0002347, OG0004869, OG0005298, OG0003677, OG0009309, OG0002019, OG0003790, OG0009183, OG0001246, OG0000174, OG0000045, OG0003013, OG0048110, OG0048111, OG0047319, OG0000879, OG0007188, OG0003847, OG0010448, OG0000352, OG0006974, OG0002646, OG0008414, OG0000082, OG0001127, OG0006264, OG0000534, OG0001181, OG0001082, OG0000575, OG0006609, OG0003772, OG0026859, OG0033389, OG0006615, OG0006232, OG0008054, OG0007774, OG0000116, OG0011706, OG0048097, OG0008815, OG0007339, OG0000119, OG0007647, OG0033387, OG0004377, OG0009082, OG0003543, OG0009102, OG0004060, OG0004521, OG0001608, OG0004226, OG0007300, OG0017602, OG0011272, OG0000077, OG0015159, OG0005372, OG0003676, OG0004786, OG0003699, OG0004144, OG0006545, OG0001316, OG0010641, OG0000048, OG0001419, OG0003900, OG0001559, OG0006125, OG0004041, OG0001279, OG0008602, OG0003998, OG0001741, OG0005614, OG0047286, OG0001188, OG0008569, OG0005822, OG0009620, OG0000239, OG0005465, OG0006172, OG0047260, OG0005633, OG0004692, OG0007653, OG0005613, OG0002487, OG0010542, OG0008027, OG0001903, OG0007461, OG0002639, OG0009150, OG0002368, OG0003360, OG0014112, OG0004751, OG0006353, OG0002028, OG0017239, OG0008422, OG0000257, OG0009058, OG0000363, OG0006385, OG0026085, OG0009475, OG0003471, OG0002875, OG0000014, OG0004884, OG0003435, OG0006849, OG0005055, OG0006149, OG0010827, OG0000724, OG0005079, OG0000128, OG0005270, OG0000391, OG0004907, OG0009724, OG0005699, OG0000696, OG0008567, OG0004202, OG0004184, OG0008673, OG0007817, OG0002761, OG0000102, OG0007314, OG0007541, OG0002376, OG0005124, OG0001018, OG0002722, OG0000548, OG0003135, OG0006225, OG0006001, OG0004644, OG0020520, OG0003004, OG0002975, OG0001673, OG0000124, OG0004407, OG0002103, OG0007177, OG0006747, OG0007277, OG0008649, OG0009723, OG0013721, OG0000312, OG0047278, OG0007309, OG0018375, OG0006373, OG0005175, OG0008311, OG0010226, OG0011256, OG0007335, OG0007450, OG0003292, OG0001490, OG0012810, OG0006136, OG0001177, OG0000429, OG0004645, OG0012872, OG0003625, OG0003859, OG0008028, OG0008767, OG0013481, OG0000121, OG0005439, OG0033134, OG0010155, OG0008658, OG0000288, OG0004082, OG0047398, OG0007304, OG0003340, OG0005283, OG0004209, OG0002414, OG0047402, OG0004014, OG0000994, OG0004228, OG0001103, OG0006259, OG0018867, OG0001463, OG0009125, OG0021810, OG0003365, OG0009396, OG0000279, OG0008580, OG0000185, OG0017301, OG0018987, OG0047412, OG0000037, OG0002267, OG0003855, OG0008809, OG0000676, OG0004757, OG0002835, OG0003370, OG0010568, OG0000325, OG0007080, OG0000175, OG0001199, OG0000527, OG0004572, OG0003679, OG0003309, OG0001344, OG0008651, OG0047742, OG0001439, OG0000214, OG0033031, OG0011442, OG0000020, OG0010154, OG0019411, OG0001722, OG0010333, OG0006702, OG0020483, OG0003670, OG0005445, OG0008682, OG0019322, OG0009216, OG0002410, OG0002740, OG0007691, OG0003536, OG0005512, OG0026163, OG0007183, OG0047882, OG0033329, OG0000341, OG0018008, OG0015529, OG0013558, OG0008510, OG0003885, OG0005950, OG0007317, OG0001857, OG0004731, OG0001864, OG0000317, OG0006379, OG0005677, OG0000434, OG0005045, OG0004942, OG0012249, OG0000247, OG0001016, OG0005855, OG0001159, OG0000293, OG0001211, OG0010890, OG0047706, OG0001753, OG0033238, OG0000031, OG0010107, OG0047708, OG0047709, OG0008009, OG0004375, OG0018953, OG0014657, OG0000567, OG0003158, OG0047115, OG0006138, OG0007042, OG0005590, OG0007827, OG0007496, OG0001661, OG0003284, OG0000523, OG0008305, OG0026053, OG0004616, OG0000390, OG0011708, OG0004622, OG0001656, OG0001328, OG0002912, OG0001400, OG0047159, OG0001633, OG0007495, OG0018083, OG0001551, OG0003005, OG0003702, OG0005150, OG0007667, OG0009675, OG0002531, OG0007981, OG0005173, OG0002555, OG0006338, OG0009164, OG0006572, OG0005837, OG0006199, OG0047280, OG0002435, OG0007968, OG0000477, OG0010610, OG0000780, OG0007392, OG0000113, OG0006003, OG0001252, OG0001730, OG0033167, OG0003507, OG0008760, OG0002193, OG0001235, OG0005900, OG0001625, OG0005506, OG0019476, OG0033376, OG0026262, OG0019029, OG0000810, OG0009335, OG0004160, OG0007119, OG0000109, OG0005144, OG0009421, OG0001670, OG0000925, OG0008519, OG0005294, OG0001986, OG0048045, OG0026170, OG0000392, OG0002164, OG0015720, OG0000732, OG0006106, OG0009092, OG0033373, OG0006316, OG0016075, OG0007793, OG0011977, OG0004860, OG0048067, OG0008872, OG0002754, OG0005191, OG0000111, OG0010545, OG0006341, OG0048068, OG0007795, OG0005277, OG0006876, OG0007847, OG0007509, OG0004389, OG0009293, OG0002789, OG0013604, OG0000588, OG0002502, OG0001337, OG0000067, OG0002562, OG0021764, OG0047671, OG0002224, OG0000972, OG0001676, OG0017364, OG0009816, OG0002212, OG0008876, OG0001858, OG0012114, OG0006990, OG0001087, OG0000400, OG0015679, OG0047676, OG0047677, OG0047678, OG0015693, OG0001360, OG0010193, OG0000727, OG0002359, OG0004296, OG0002196, OG0001493, OG0007075, OG0003388, OG0003813, OG0033230, OG0002655, OG0000316, OG0001936, OG0000218, OG0003383, OG0009424, OG0007220, OG0003492, OG0001820, OG0006276, OG0003355, OG0000063, OG0000034, OG0009824, OG0021840, OG0010181, OG0000188, OG0009152, OG0002153, OG0003176, OG0008857, OG0004921, OG0007661, OG0005766, OG0009135, OG0005142, OG0005895, OG0000326, OG0006745, OG0003716, OG0000313, OG0007338, OG0002948, OG0005242, OG0001404, OG0006683, OG0003925, OG0015535, OG0013496, OG0005418, OG0011721, OG0002779, OG0008547, OG0008079, OG0000953, OG0026255, OG0007122, OG0004121, OG0002522, OG0013239, OG0025898, OG0018068, OG0002058, OG0007806, OG0000572, OG0011766, OG0001960, OG0003926, OG0011111, OG0002418, OG0006983, OG0004808, OG0001869, OG0011654, OG0000894, OG0001410, OG0005750, OG0000997, OG0002712, OG0018973, OG0000337, OG0002537, OG0012840, OG0006537, OG0019357, OG0018162, OG0001233, OG0001287, OG0004402, OG0008328, OG0001704, OG0007006, OG0007044, OG0021812, OG0008075, OG0010104, OG0021772, OG0011698, OG0011672, OG0000310, OG0016436, OG0000007, OG0001871, OG0002401, OG0008977, OG0000322, OG0007452, OG0047857, OG0005570, OG0006668, OG0000019, OG0047860, OG0031954, OG0004707, OG0006365, OG0004587, OG0006881, OG0007298, OG0008432, OG0002622, OG0002095, OG0009165, OG0005423, OG0003157, OG0007800, OG0026226, OG0001519, OG0003294, OG0013488, OG0033314, OG0007010, OG0004697, OG0011168, OG0003953, OG0004781, OG0007628, OG0008043, OG0047849, OG0019213, OG0003011, OG0017282, OG0001186, OG0005609, OG0007929, OG0006262, OG0005135, OG0047448, OG0004482, OG0005245, OG0001396, OG0012225, OG0001095, OG0000895, OG0001956, OG0047453, OG0000406, OG0010920, OG0005235, OG0003884, OG0004471, OG0005272, OG0002452, OG0016427, OG0003481, OG0010861, OG0000848, OG0005979, OG0000006, OG0003134, OG0004758, OG0000142, OG0002517, OG0002149, OG0005413, OG0015531, OG0005412, OG0007633, OG0005383, OG0004628, OG0006165, OG0007527, OG0002787, OG0002491, OG0001666, OG0004403, OG0001678, OG0002336, OG0000619, OG0001790, OG0004627, OG0008280, OG0011759, OG0001584, OG0047486, OG0007089, OG0001555, OG0001747, OG0047488, OG0002725, OG0002459, OG0008866, OG0005373, OG0005007, OG0000084, OG0000948, OG0007773, OG0008257, OG0001139, OG0002998, OG0006993, OG0003047, OG0004803, OG0002403, OG0001511, OG0001719, OG0000731, OG0000364, OG0008323, OG0005795, OG0007497, OG0007400, OG0000256, OG0003054, OG0047520, OG0047499, OG0001401, OG0007070, OG0023006, OG0005714, OG0001001, OG0006094, OG0009043, OG0006256, OG0026187, OG0001496, OG0033243, OG0047087, OG0005103, OG0006389, OG0000137, OG0010223, OG0014825, OG0002293, OG0000209, OG0002841, OG0005040, OG0012719, OG0000090, OG0005448, OG0000716, OG0012716, OG0002063, OG0013613, OG0026025, OG0006387, OG0007672, OG0000040, OG0001668, OG0014127, OG0003189, OG0002629, OG0006960, OG0002879, OG0000638, OG0004820, OG0007399, OG0002499, OG0001780, OG0000074, OG0033032, OG0002135, OG0001170, OG0008595, OG0008275, OG0001554, OG0002685, OG0006694, OG0011208, OG0007284, OG0003549, OG0047110, OG0007848, OG0000890, OG0000909, OG0009839, OG0047086, OG0011032, OG0033127, OG0002022, OG0001306, OG0006686, OG0019509, OG0001412, OG0003376, OG0000254, OG0001613, OG0048026, OG0002656, OG0012829, OG0016442, OG0004935, OG0001743, OG0004710, OG0002070, OG0008317, OG0003557, OG0001314, OG0004345, OG0001802, OG0000453, OG0000064, OG0007660, OG0009093, OG0001305, OG0007155, OG0008318, OG0017006, OG0000529, OG0003210, OG0018984, OG0003868, OG0003036, OG0013770, OG0000412, OG0000236, OG0007329, OG0000151, OG0009658, OG0003737, OG0002318, OG0009568, OG0005974, OG0009129, OG0000574, OG0006376, OG0006570, OG0005522, OG0008947, OG0009168, OG0005919, OG0002628, OG0002751, OG0002217, OG0000592, OG0006987, OG0009182, OG0004909, OG0007239, OG0002855, OG0008276, OG0001571, OG0005201, OG0007417, OG0013029, OG0006908, OG0002508, OG0000201, OG0007820, OG0007316, OG0003872, OG0005857, OG0007487, OG0000323, OG0004514, OG0001582, OG0001220, OG0007736, OG0001914, OG0009544, OG0006308, OG0008524, OG0005488, OG0002799, OG0001451, OG0005377, OG0047192, OG0008284, OG0005816, OG0003128, OG0003387, OG0005815, OG0007478, OG0009565, OG0008411, OG0006634, OG0001525, OG0002479, OG0005915, OG0008870, OG0004776, OG0000721, OG0001799, OG0047174, OG0005321, OG0009411, OG0008074, OG0002297, OG0010956, OG0009571, OG0003639, OG0003291, OG0000344, OG0002344, OG0009582, OG0002211, OG0000943, OG0000643, OG0006604, OG0009180, OG0008837, OG0001164, OG0002728, OG0004988, OG0005535, OG0000800, OG0000168, OG0047166, OG0007775, OG0004789, OG0000847, OG0008797, OG0003927, OG0004788, OG0002166, OG0004995, OG0009195, OG0004914, OG0006096, OG0006010, OG0001720, OG0033075, OG0012827, OG0000241, OG0001216, OG0001234, OG0008046, OG0004903, OG0000402, OG0003371, OG0011266, OG0007243, OG0002354, OG0003251, OG0004216, OG0002908, OG0007324, OG0006513, OG0003769, OG0019446, OG0004460, OG0002034, OG0007253, OG0006180, OG0002590, OG0011764, OG0004312, OG0007111, OG0012858, OG0047245, OG0000612, OG0034480, OG0001487, OG0009140, OG0000115, OG0011207, OG0001405, OG0008496, OG0000546, OG0002461, OG0012180, OG0002421, OG0002716, OG0001868, OG0000235, OG0006098, OG0008861, OG0007160, OG0007745, OG0003480, OG0016997, OG0002668, OG0004872, OG0006118, OG0004842, OG0001572, OG0003048, OG0024547, OG0002201, OG0007114, OG0011718, OG0006100, OG0003574, OG0000978, OG0009485, OG0009587, OG0000359, OG0000273, OG0009091, OG0005524, OG0002083, OG0005898, OG0007424, OG0004527, OG0001953, OG0008389, OG0000781, OG0007836, OG0006212, OG0000056, OG0008598, OG0047335, OG0047313, OG0013674, OG0007048, OG0047339, OG0005651, OG0009896, OG0000818, OG0000777, OG0006678, OG0007601, OG0000311, OG0003567, OG0006566, OG0002902, OG0005701, OG0001883, OG0008350, OG0002724, OG0002433, OG0007977, OG0001776, OG0006070, OG0015528, OG0033109, OG0002234, OG0017003, OG0003356, OG0005395, OG0008588, OG0005831, OG0000297, OG0032781, OG0004405, OG0004125, OG0004504, OG0002602, OG0012844, OG0002090, OG0005208, OG0047325, OG0007019, OG0000046, OG0004252, OG0008577, OG0004738, OG0007479, OG0004883, OG0007801, OG0002286, OG0006642, OG0000623, OG0002228, OG0000083, OG0000431, OG0000159, OG0008254, OG0004340, OG0004605, OG0005134, OG0011055, OG0002888, OG0002666, OG0004559, OG0001452, OG0013656, OG0003330, OG0006243, OG0015166, OG0003854, OG0007457, OG0008252, OG0005752, OG0026896, OG0000573, OG0010209, OG0008227, OG0011694, OG0005583, OG0001136, OG0047622, OG0009935, OG0008250, OG0011241, OG0014001, OG0001614, OG0004270, OG0008302, OG0023995, OG0001030, OG0015805, OG0006770, OG0003115, OG0011774, OG0002042, OG0006015, OG0011635, OG0002246, OG0020445, OG0003239, OG0004477, OG0002295, OG0005824, OG0004960, OG0014681, OG0033045, OG0002853, OG0009611, OG0001768, OG0008875, OG0003820, OG0003470, OG0047628, OG0001031, OG0004311, OG0000679, OG0006302, OG0001503, OG0008016, OG0001728, OG0026199, OG0026211, OG0007176, OG0009633, OG0007058, OG0010251, OG0047818, OG0000183, OG0008581, OG0003390, OG0007097, OG0003266, OG0000242, OG0001194, OG0047794, OG0009580, OG0004944, OG0002490, OG0005429, OG0007808, OG0000858, OG0015744, OG0008394, OG0001495, OG0007928, OG0001632, OG0005475, OG0006342, OG0002750, OG0001079, OG0007469, OG0006297, OG0002756, OG0006322, OG0005937, OG0004349, OG0003666, OG0008094, OG0009872, OG0011294, OG0000774, OG0000360, OG0006550, OG0001270, OG0004547, OG0005600, OG0007516, OG0009314, OG0014584, OG0014695, OG0000536, OG0017189, OG0004244, OG0008864, OG0002715, OG0006933, OG0005086, OG0003265, OG0003441, OG0008011, OG0000861, OG0001247, OG0003447, OG0047966, OG0001852, OG0003514, OG0007909, OG0033353, OG0000664, OG0010130, OG0003842, OG0021853, OG0004621, OG0005659, OG0002155, OG0002973, OG0000097, OG0021836, OG0003358, OG0006997, OG0002989, OG0005786, OG0001356, OG0006676, OG0005568, OG0001369, OG0005599, OG0000415, OG0002986, OG0005772, OG0007610, OG0000790, OG0001663, OG0018205, OG0001232, OG0001317, OG0002209, OG0014490, OG0000456, OG0001643, OG0047723, OG0006053, OG0002545, OG0024464, OG0005625, OG0007170, OG0003611, OG0009430, OG0010930, OG0000601, OG0006363, OG0007778, OG0006167, OG0006982, OG0003320, OG0003368, OG0001378, OG0007823, OG0002565, OG0003554, OG0000850, OG0004343, OG0008381, OG0016407, OG0005955, OG0004459, OG0003948, OG0005195, OG0009666, OG0000282, OG0002235, OG0007732, OG0005603, OG0008301, OG0006890, OG0003177, OG0009157, OG0008222, OG0003548, OG0007649, OG0002312, OG0010914, OG0004245, OG0001606, OG0009174, OG0047591, OG0004362, OG0009584, OG0007347, OG0008644, OG0003147, OG0001798, OG0002084, OG0000298, OG0001756, OG0002321, OG0016513, OG0006119, OG0005119, OG0047607, OG0001510, OG0006679, OG0008698, OG0008320, OG0003863, OG0005836, OG0000662, OG0006919, OG0011702, OG0002285, OG0009813, OG0001627, OG0006250, OG0047614, OG0011692, OG0009451, OG0008364, OG0002737, OG0047745, OG0000586, OG0026193, OG0006614, OG0019017, OG0001361, OG0003778, OG0004824, OG0000135, OG0004294, OG0012241, OG0011367, OG0009112, OG0000300, OG0016416, OG0010518, OG0011596, OG0007074, OG0001023, OG0007612, OG0002994, OG0004746, OG0007219, OG0000872, OG0013589
## gfams_excl_b
## 2 OG0049752, OG0005739, OG0030846, OG0014595, OG0049753, OG0005523, OG0004571, OG0018356, OG0049760, OG0006551, OG0024749, OG0003805, OG0002333, OG0010197, OG0003913, OG0005088, OG0009647, OG0000055, OG0001696, OG0010918, OG0003363, OG0019451, OG0005716, OG0006930, OG0008099, OG0005768, OG0007939, OG0010135, OG0005756, OG0009156, OG0021038, OG0002945, OG0011317, OG0004475, OG0024737, OG0002038, OG0000187, OG0004916, OG0001162, OG0004963, OG0002481, OG0000104, OG0000009, OG0002967, OG0000553, OG0000513, OG0000817, OG0000136, OG0001411, OG0005286, OG0002512, OG0009406, OG0014608, OG0007714, OG0008242, OG0002997, OG0006156, OG0008006, OG0005214, OG0002140, OG0006313, OG0003200, OG0022858, OG0008026, OG0003738, OG0003449, OG0005745, OG0014531, OG0001477, OG0002072, OG0009910, OG0003109, OG0006227, OG0005428, OG0009144, OG0011613, OG0015794, OG0002770, OG0001339, OG0005948, OG0004048, OG0024833, OG0002834, OG0030997, OG0000107, OG0000043, OG0009096, OG0015297, OG0005137, OG0000234, OG0014209, OG0008373, OG0004617, OG0004574, OG0044209, OG0031677, OG0000594, OG0009865, OG0010153, OG0019520, OG0005604, OG0024797, OG0005971, OG0031822, OG0004800, OG0005455, OG0003923, OG0018685, OG0013333, OG0000053, OG0007833, OG0000566, OG0000017, OG0005385, OG0011641, OG0042957, OG0000264, OG0030877, OG0000324, OG0021033, OG0006586, OG0004845, OG0027026, OG0001762, OG0016819, OG0002648, OG0003220, OG0007283, OG0002637, OG0000276, OG0001121, OG0004139, OG0042958, OG0003060, OG0006559, OG0002189, OG0000382, OG0018517, OG0003728, OG0006241, OG0004495, OG0005507, OG0001363, OG0016665, OG0008772, OG0004568, OG0004291, OG0003500, OG0003455, OG0002213, OG0005367, OG0006300, OG0007746, OG0027115, OG0009607, OG0006767, OG0007642, OG0005203, OG0030829, OG0001123, OG0004829, OG0001446, OG0004956, OG0004018, OG0000073, OG0008269, OG0003353, OG0008265, OG0007275, OG0008878, OG0004659, OG0006335, OG0014612, OG0021001, OG0013601, OG0004996, OG0000122, OG0000923, OG0010954, OG0008402, OG0005711, OG0000091, OG0012214, OG0000085, OG0044572, OG0003448, OG0006526, OG0005248, OG0010179, OG0007315, OG0002671, OG0004965, OG0001029, OG0031768, OG0005305, OG0049949, OG0000065, OG0000069, OG0049952, OG0030959, OG0009682, OG0000569, OG0000029
## 3 OG0000808, OG0000253, OG0024758, OG0031798, OG0004764, OG0000095, OG0024773, OG0000105, OG0000149, OG0024753, OG0017327, OG0001121, OG0005081, OG0025445, OG0014691, OG0010529, OG0013706, OG0003597, OG0000055, OG0000107, OG0012368, OG0000086, OG0024191, OG0008693, OG0000088, OG0020996, OG0010543, OG0007703, OG0010985, OG0010567, OG0008203, OG0002156, OG0005159, OG0001839, OG0012170, OG0042971, OG0000346, OG0006761, OG0022404, OG0001061, OG0003040, OG0003898, OG0009156, OG0015298, OG0015835, OG0030991, OG0006328, OG0000663, OG0001637, OG0044833, OG0005417, OG0008513, OG0003773, OG0006008, OG0005510, OG0001182, OG0049752, OG0030925, OG0025446, OG0002073, OG0000029, OG0049857, OG0021031, OG0012855, OG0016794, OG0000452, OG0012291, OG0004753, OG0010926, OG0000180, OG0002838, OG0044233, OG0019143, OG0014281, OG0013695, OG0000001, OG0007982, OG0002477, OG0009250, OG0000420, OG0030874, OG0004021, OG0006621, OG0049760, OG0001762, OG0000052, OG0007651, OG0014692, OG0002968, OG0011528, OG0006929, OG0049753, OG0008050, OG0031721, OG0044886, OG0031934, OG0000694, OG0003462, OG0005324, OG0004778, OG0007922, OG0015299, OG0005146, OG0005884, OG0013670, OG0001478, OG0015297, OG0002558, OG0044708, OG0000110, OG0024719, OG0005905, OG0013661, OG0003285, OG0000000, OG0004519, OG0002333, OG0026995, OG0021001, OG0006312, OG0004996, OG0010197, OG0008758, OG0000069, OG0002219, OG0010507, OG0015606, OG0007146, OG0027402, OG0010548, OG0000141, OG0007449, OG0007604, OG0014724, OG0008224, OG0024703, OG0006079, OG0000169
## 4 OG0009557, OG0000648, OG0008699, OG0049762, OG0003285, OG0014237, OG0003596, OG0012788, OG0000052, OG0007305, OG0015375, OG0000292, OG0008001, OG0000345, OG0000324, OG0010604, OG0000073, OG0012027, OG0000956, OG0002370, OG0000533, OG0000076, OG0000055, OG0017121, OG0011141, OG0000871, OG0002647, OG0009947, OG0006086, OG0011652, OG0000043, OG0000017, OG0002273, OG0009639, OG0012368, OG0001336, OG0000080, OG0026530, OG0001149, OG0005684, OG0001835, OG0000009, OG0001601, OG0007507, OG0011191, OG0005006, OG0000134, OG0005457, OG0004876, OG0019129, OG0000132, OG0010989, OG0013281, OG0014738, OG0011371, OG0014188, OG0011821, OG0014072, OG0010617, OG0015872
## 5 OG0000564, OG0006527, OG0010537, OG0024852, OG0000182, OG0019835, OG0016034, OG0000076, OG0010868, OG0009548, OG0000098, OG0000187, OG0016819, OG0000747, OG0012335, OG0001301, OG0001588, OG0001995, OG0000340, OG0000207, OG0000005, OG0010504, OG0011191, OG0004876, OG0003901, OG0003596, OG0033901, OG0000009, OG0014364, OG0000937, OG0002417, OG0002796, OG0000017, OG0000088, OG0005063, OG0003285, OG0000052, OG0001909, OG0000924, OG0000107, OG0012754, OG0000050, OG0001061, OG0012788, OG0000630, OG0012027, OG0000110, OG0007760, OG0011288, OG0019217, OG0014555, OG0033889, OG0013331, OG0012063, OG0000029, OG0015794, OG0008690, OG0008371, OG0000169, OG0009859, OG0010121, OG0000571, OG0002055, OG0000203, OG0010986, OG0034107, OG0001099, OG0004537, OG0049879, OG0000026, OG0013537, OG0000178, OG0006056, OG0015154, OG0006704, OG0042904, OG0000153, OG0009869, OG0000004, OG0033189, OG0049908, OG0009682, OG0019591, OG0009367, OG0014692, OG0000118, OG0031998, OG0024745, OG0011777, OG0007969, OG0006274, OG0007520, OG0007950, OG0001443, OG0031904, OG0002960, OG0000348, OG0049762, OG0044481, OG0000399, OG0000560, OG0000032, OG0000581, OG0017357, OG0000350, OG0002023, OG0012033, OG0042960, OG0026519, OG0003614, OG0012143, OG0012917, OG0019629, OG0002849, OG0000012, OG0000104, OG0011826, OG0018500, OG0000330, OG0003427, OG0000047, OG0005579, OG0000078, OG0000075, OG0001935, OG0009380, OG0000132, OG0026534, OG0002442, OG0001320, OG0025403, OG0006129, OG0004053, OG0000535, OG0011334
## 6 OG0000017, OG0000024, OG0001647, OG0006473, OG0000808, OG0021017, OG0017118, OG0014389, OG0003596, OG0025490, OG0001703, OG0008485, OG0008436, OG0014197, OG0000038, OG0000747, OG0010819, OG0033889, OG0007259, OG0000042, OG0003285, OG0019131, OG0003991, OG0009429, OG0002373, OG0004006, OG0000789, OG0012146, OG0001229, OG0008178, OG0024815, OG0000114, OG0044796, OG0001061, OG0004777, OG0013436, OG0000830, OG0022048, OG0009324, OG0042909, OG0000081, OG0000182, OG0002178, OG0026505, OG0001759, OG0009966, OG0002823, OG0000023, OG0000000, OG0000029, OG0011141, OG0007232, OG0049862, OG0000169, OG0000399, OG0000711, OG0012610, OG0000080, OG0004418, OG0011138, OG0008534, OG0026516, OG0031821, OG0044820, OG0000959, OG0016039, OG0000423, OG0000988, OG0008410, OG0015607, OG0000519, OG0015603, OG0014243, OG0015641, OG0004651, OG0000033, OG0001099, OG0015311, OG0000207, OG0001844, OG0001336, OG0018678, OG0007579, OG0000161, OG0004036, OG0019484, OG0014551, OG0006900, OG0000541, OG0021047, OG0010085, OG0000253, OG0000728, OG0005244, OG0011531, OG0018728, OG0024853, OG0010627, OG0002817, OG0009909, OG0015304, OG0011701, OG0030841, OG0000015, OG0004344, OG0030890, OG0006847, OG0013328, OG0022043, OG0030770, OG0005084, OG0003637, OG0003209, OG0010171, OG0000324, OG0014812, OG0003899, OG0001386, OG0000238, OG0013984, OG0030938, OG0001443, OG0008221, OG0003153, OG0006647, OG0012257, OG0000013, OG0002591
## 7 OG0009308, OG0004876, OG0007999, OG0004875, OG0017799, OG0008134, OG0000086, OG0001909, OG0003085, OG0011191, OG0002546, OG0006751, OG0002313, OG0002309, OG0000017, OG0000348, OG0000134, OG0004575, OG0001954, OG0002606, OG0001690, OG0001995, OG0000127, OG0004522, OG0009130, OG0000483, OG0005349, OG0000100, OG0001598, OG0006042, OG0000788, OG0019136, OG0000549, OG0009638, OG0000497, OG0000065, OG0019315, OG0010534, OG0001662, OG0000845, OG0000029, OG0006835, OG0006307, OG0000659, OG0000050, OG0000637, OG0000292, OG0004426, OG0012247, OG0000002, OG0044537, OG0044653, OG0001301, OG0022451, OG0003908, OG0000059, OG0000263, OG0000000, OG0000013, OG0012754, OG0033953, OG0006393, OG0002607A look at the number of genes belonging to enriched gene families between comparisons
# Group by module and summarize
commongenes <- PFLA_SPUR_COMPARISON_OF$orthology_overlap_modules$
genes_in_common_fams$commonfams$table_a_common %>%
group_by(module) %>%
summarize(
numgenes = n(),
genes = paste(ifelse(n() > 3, paste0(paste(head(id, 3), collapse = ",")," ..."), paste(id, collapse = ", ")), collapse = ", ")
)
# Rename columns
colnames(commongenes) <- c("module", "numgenes", "genes")
commongenes## # A tibble: 20 × 3
## module numgenes genes
## <chr> <int> <chr>
## 1 14__16 121 TCONS_00023217,TCONS_00023345,TCONS_00024858 ...
## 2 15__13 107 TCONS_00031733,TCONS_00032648,TCONS_00038402 ...
## 3 15__21 103 TCONS_00031733,TCONS_00032648,TCONS_00054720 ...
## 4 15__24 146 TCONS_00054720,TCONS_00024740,TCONS_00025053 ...
## 5 17__15 126 TCONS_00038723,TCONS_00032297,TCONS_00032533 ...
## 6 17__21 168 TCONS_00038723,TCONS_00032270,TCONS_00032272 ...
## 7 17__24 199 TCONS_00038723,TCONS_00032270,TCONS_00032272 ...
## 8 17__25 270 TCONS_00038723,TCONS_00032270,TCONS_00032272 ...
## 9 19__15 132 TCONS_00024521,TCONS_00023416,TCONS_00023644 ...
## 10 19__21 189 TCONS_00032267,TCONS_00009468,TCONS_00024521 ...
## 11 20__15 91 TCONS_00025254,TCONS_00023950,TCONS_00025325 ...
## 12 20__21 154 TCONS_00032259,TCONS_00032260,TCONS_00032261 ...
## 13 20__24 190 TCONS_00032259,TCONS_00032260,TCONS_00032261 ...
## 14 20__25 266 TCONS_00032259,TCONS_00032260,TCONS_00032261 ...
## 15 21__15 87 TCONS_00052333,TCONS_00023006,TCONS_00024151 ...
## 16 21__21 125 TCONS_00032266,TCONS_00023006,TCONS_00025060 ...
## 17 22__15 66 TCONS_00032247,TCONS_00024155,TCONS_00024279 ...
## 18 22__21 123 TCONS_00039101,TCONS_00039119,TCONS_00032247 ...
## 19 22__23 112 TCONS_00025536,TCONS_00025537,TCONS_00056380 ...
## 20 22__24 148 TCONS_00039101,TCONS_00039119,TCONS_00024155 ...The gene age enrichment and functional category enrichment of these genes:
PFLA_SPUR_COMPARISON_OF$orthology_overlap_modules$
genes_in_common_fams$commonfams$
age_a_common$heatmap +
PFLA_SPUR_COMPARISON_OF$orthology_overlap_modules$
genes_in_common_fams$commonfams$
cog_a_comon$heatmap
And the GO terms of these genes:
# Add a new column with the name of the original data frame
keygenes_commonfams_GO <-
bind_rows(
PFLA_SPUR_COMPARISON_OF$orthology_overlap_modules$
genes_in_common_fams$commonfams$go_a_common$GOtable,
.id = "pair_gfams"
)
# Rename the new column
colnames(keygenes_commonfams_GO)[1] <- "pair_gfams"
keygenes_commonfams_GO$logp <-
-log10(as.numeric(keygenes_commonfams_GO$classicFisher))
keygenes_commonfams_GO$logp[is.na(keygenes_commonfams_GO$logp)] <-
max(keygenes_commonfams_GO$logp, na.rm = T)
keygenes_commonfams_GO$obsexp <-
as.numeric(keygenes_commonfams_GO$Significant/keygenes_commonfams_GO$Expected)
keygenes_commonfams_GO <-
keygenes_commonfams_GO[
order(
keygenes_commonfams_GO$pair_gfams,
keygenes_commonfams_GO$Term
),
]
keygenes_commonfams_GO$Term <-
factor(
keygenes_commonfams_GO$Term,
levels = unique(keygenes_commonfams_GO$Term)
)The plot of the GO enrichment analysis:
gg <- keygenes_commonfams_GO %>%
ggplot(
aes(x=pair_gfams, y = Term, size = Significant, color = logp)
) + geom_point() +
theme_bw() +
theme(
axis.text.x = element_text(angle = 45, hjust = 1, size = 8 )
)+
theme(
axis.text.y.left = element_text(hjust = 1, size = 8 ),
legend.title=element_text(size=8 ),
legend.text=element_text(size=8 )
)+
scale_size_continuous(
name = "Genes affected",
range = c(2,6)
) + scale_color_viridis("-log10(P-value)") +
xlab("")+ylab("")
#ggSaving the data
We will save these two objects for further analysis and reference:
save(
PFLA_BLAN_COMPARISON_OF,
PFLA_SPUR_COMPARISON_OF,
file = "outputs/rda/species_comparison_orthofinder.rda"
)